Department of Critical Care Medicine, Taichung Veterans General Hospital
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 200
- 试验地点
- 1
- 主要终点
- The dynamics of the airway bacterial microbiome in the lower respiratory tract specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation)
研究概览
简要总结
A prospective observational study. Enrolled participants admitted to ICU due to pneumonia and respiratory failure need mechanical ventilator support. Investigators collected the residual specimens, such as sputum from endotrachea aspiration, bronchoalveolar lavage fluid in those participants as the usual care in the ICU. Those residual samples were sent to extract RNA and sequence by using high-throughput sequencing (next-generation sequencing) method.
Investigators will compared the microbiome feature between lower respiratory tract and stool specimens in those participants diagnosed as pneumonia with respiratory failure.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 40 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adults aged than 40 years old
- •Diagnosed as pneumonia
- •Respiratory failure with mechanical ventilator support
排除标准
- •Less than 40 years old
- •Has been treated with antibiotics more than 7 days within two weeks before enrolling into study
- •Suspected or active pulmonary tuberculosis infection
结局指标
主要结局
The dynamics of the airway bacterial microbiome in the lower respiratory tract specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation)
时间窗: Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7
Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S rRNA sequence of bacteria in residual samples
次要结局
- The dynamics of the gut bacterial microbiome in the stool specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation)(Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7)
- The association of the microbiome between lower respiratory tracts and stool specimens in pneumonic patient with respiratory failure(Compared the association of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7)
研究者
Pin-Kuei Fu, MD, PhD
Principal Investigator, Department of Critical Care Medicine
Taichung Veterans General Hospital