Effect of Embryo Biopsy Side on Post Biopsy Blastocyst Structurs and Implantation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 100
- 试验地点
- 4
- 主要终点
- blastocyst location of ICM at day 5
研究概览
简要总结
Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.
详细描述
During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated.
Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer.
In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality.
排除标准
- •grade 3 embryo at day 3, or 8-3 embryos.
研究组 & 干预措施
Vegetal Pole blastomer embryo
for day 3 embryo biopsy / cleavage stage: Blastomere collection from opposite side to the 2nd polar body or from vegetal pole.
干预措施: Vegetal Pole blastomer embryo (Procedure)
Vegetal Pole blastomer embryo
for day 3 embryo biopsy / cleavage stage: Blastomere collection from opposite side to the 2nd polar body or from vegetal pole.
干预措施: Animal Pole blastomer embryo (Procedure)
Animal Pole blastomer embryo
for day 3 embryo biopsy / cleavage stage: Blastomere collection from and side around the 2nd polar body and not the vegetal pole.
干预措施: Vegetal Pole blastomer embryo (Procedure)
Animal Pole blastomer embryo
for day 3 embryo biopsy / cleavage stage: Blastomere collection from and side around the 2nd polar body and not the vegetal pole.
干预措施: Animal Pole blastomer embryo (Procedure)
结局指标
主要结局
blastocyst location of ICM at day 5
时间窗: 2 months
describing the location of the inner cell mass (ICM) in location to the zona peluceda (ZP) drilling opening.
次要结局
- Implantation power(2 months)
研究者
Ahmad Mustafa Mohamed Metwalley
IVF laboratory manager
Al Baraka Fertility Hospital