Viral and Microbial Circulation Between Humans, Domesticated and Wild Animals Along an Ecotone, Democratic Republic of Congo
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Percentage of gut bacterial genera shared by selected wild animals
研究概览
简要总结
This study will evaluate the overlap between the intestinal microbiome and virome of wild and domesticated animals and human beings living in close proximity in three sites along an ecotone (ecological gradient) in the Democratic Republic of Congo.
详细描述
The MICROTONE study sheds light on zoonotic disease emergence by examining social and ecological pathways facilitating microbial and viral flows between people and selected wild and domesticated animals along a gradient of ecological change in a forest-savanna mosaic in the Democratic Republic of Congo, an epicenter of zoonotic disease emergence. The investigators analyze potential viral and bacterial overlap among humans and animals and explain this overlap (or not) through social sciences and ecological analyses of human and animal mobilities, practices and contacts. This multi-disciplinary, multi-species investigation in an ecotone (a transitional ecological zone linked to zoonotic disease emergence) offers a "pre-history" of spillover and emergence, tracing an ecological zeb of virome and microbial sharing among humans and animals. It will elucidate why such microbial and viral flows occur.
To conduct this investigation,there are two human sub-studies: the social sciences participatory study; and the clinical study. The clinical study will involve 30 human subjects from whom blood and stool samples will be collected. The social sciences participatory study will involve self-collected activity and animal contact data and oral interviews among 60 human subjects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 18 years or older
- •Accept participation in study
- •Residing in one of three villages selected
- •In good health and able to conduct habitual daily activities
- •Accept self-collection of 2g of stool
- •Accept blood collection (2-2.5 ml) by a medical professional
- •Accept to self-collect daily activities and contacts with selected wild and domesticated animals for five months
- •Accept to participate in an anthropological-historical interview on changing practices and contacts with wild and domesticated animals
排除标准
- •Vulnerable adults will not be included
- •Women who are pregnant or nursing at the time of recruitment and inclusion will not be included.
- •Adults declaring themselves ill and unable to conduct their habitual daily activities will not be included.
- •Adults with a chronic illness will not be included
- •Adults who have a family member already included in the study will not be included.
- •Adults refusing either recording of interviews or note-taking during interviews will not be included.
研究组 & 干预措施
Microbiological, anthropological and historical study
30 adult participants will be recruited to self-report daily activities and contacts with domesticated and wild animals for a five month period. Following the five months of data collection, we will collect 5ml blood and 2g stool from each participant.
From the fifth month of investigation, an additional 30 adult participants will participate in oral anthropological, historical interviews to develop the socio-historical context of their changing activities and relations with selected domesticated and wild animals. No other intervention will be performed.
干预措施: Anthropological and historical interviews (Other)
Microbiological, anthropological and historical study
30 adult participants will be recruited to self-report daily activities and contacts with domesticated and wild animals for a five month period. Following the five months of data collection, we will collect 5ml blood and 2g stool from each participant.
From the fifth month of investigation, an additional 30 adult participants will participate in oral anthropological, historical interviews to develop the socio-historical context of their changing activities and relations with selected domesticated and wild animals. No other intervention will be performed.
干预措施: blood sampling (Biological)
Microbiological, anthropological and historical study
30 adult participants will be recruited to self-report daily activities and contacts with domesticated and wild animals for a five month period. Following the five months of data collection, we will collect 5ml blood and 2g stool from each participant.
From the fifth month of investigation, an additional 30 adult participants will participate in oral anthropological, historical interviews to develop the socio-historical context of their changing activities and relations with selected domesticated and wild animals. No other intervention will be performed.
干预措施: stool collection (Biological)
Microbiological, anthropological and historical study
30 adult participants will be recruited to self-report daily activities and contacts with domesticated and wild animals for a five month period. Following the five months of data collection, we will collect 5ml blood and 2g stool from each participant.
From the fifth month of investigation, an additional 30 adult participants will participate in oral anthropological, historical interviews to develop the socio-historical context of their changing activities and relations with selected domesticated and wild animals. No other intervention will be performed.
干预措施: Participatory activity and contact investigation (Other)
结局指标
主要结局
Percentage of gut bacterial genera shared by selected wild animals
时间窗: 2 years (March 13, 2019 to March 13, 2021)
The investigators will evaluate the gut bacteria shared by wild animals on a daily basis.
Percentage of gut bacterial genera shared by included people
时间窗: 2 years (March 13, 2019 to March 13, 2021)
The investigators will evaluate the gut bacteria shared by humans on a daily basis.
Percentage of gut bacterial genera shared by selected domesticated animals
时间窗: 2 years (March 13, 2019 to March 13, 2021)
The investigators will evaluate the gut bacteria shared by domesticated animals living in proximity on a daily basis.
Percentage of overlap (of viral genera) between human subjects and great apes
时间窗: 2 years (March 13, 2019 to March 13, 2021)
The percentage of overlap (of viral genera) between human subjects and great apes will be measured.
次要结局
- Description of Contact type(2 years (March 13,2019 to March 13, 2021))
- Contact frequency investigation(2 years (March 13, 2019 to March 13, 2021))