Comparison of Effectiveness of Sodium Hypochlorite and Chlorhexidine Gluconate for Disinfection of Gutta Percha Cones
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 发起方
- 入组人数
- 124
- 试验地点
- 1
- 主要终点
- effectiveness of disinfectants by assessing turbidity in broth using mann whitney test
研究概览
简要总结
This is a comparative experimental study to compare the efficacy of chemical disinfection agents , sodium hypochlorite and chlorhexidine gluconate in decontaminating gutta percha cones
详细描述
The study design is a comparative study which was done to compare the efficacy of chemical disinfection agents on decontaminating the gutta percha cones .
In this study, 124 standardized size-80 gutta-percha (GP) cones (Dentsply, Germany) were used to assess the efficacy of chemical disinfectants.
The cones were first sterilized using ethylene oxide to ensure baseline sterility. Following sterilization, 124 cones were allocated into two experimental groups (n = 62 each) for contamination.
group A . gp were immersed in 20ml of Enterococcus faecalis solution group B . gp were immersed in 20 ml of human saliva in patients undergoing root canal therapy
samples were incubated at 37°C for 72 hours to ensure uniform and thorough contamination
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Triple (Participant, Care Provider, Investigator)
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •healthy individual age between 20-40 patient needed root canal therapy
排除标准
- •patient not willing to participate in study participants with systemic disease patients with neuromuscular disorder
研究组 & 干预措施
Naocl group
In this group, the Gp cones would be submerged in 5% naocl solution for 5 minutes and then tested for biofilm removal and eradication of inoculated microorganisms.
干预措施: Sodium Hypochlorite (Drug)
chlorhexidine group
In this group, the Gp cones would be submerged in 0.2% chlorhexidine solution for 5 minutes and then tested for biofilm removal and eradication of inoculated microorganisms.
干预措施: Chlorhexidine (Drug)
结局指标
主要结局
effectiveness of disinfectants by assessing turbidity in broth using mann whitney test
时间窗: 24 weeks
Following disinfection, all gutta-percha (GP) cones were dried again with sterile gauze and placed individually into sterile test tubes containing 20 mL of fresh brain heart infusion (BHI) broth. The test tubes were incubated at 37°C for 72 hours. The presence of bacterial growth was determined by assessing turbidity in the broth, indicating viable microorganisms. Quantitative analysis of turbidity data was performed using the Mann-Whitney test and Kruskal-Wallis test to evaluate the effectiveness of the disinfectants across the groups.
次要结局
未报告次要终点
研究者
Hira Amjad
Dr. Hafiza Hira Amjad
Armed Forces Institute of Dentistry, Pakistan