The Evaluation of Thiol Redox Status in Conjunctiva of Dry Eye Patients
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Tear amount, tear break up time, and so on are measured clinically. The thiol redox status is measured by staining the membrane.
研究概览
简要总结
Dry eye disease is related with oxidative stress at the ocular surface. GSH is one of the key factor of protect from oxidative stress. To evaluate thiol status of ocular surface cells by monitoring GSH concentration, we can evaluate the severity of dry eye.
详细描述
The conjunctival cells are collected by impression cytology. Those cells are stained with 100 mM of monochlorobimane (MCB, M-1381, Molecular Probes, Eugene, OR) and propidium iodide (PI). Fluorescent intensity, reflecting the amount of icGSH, was inspected under a confocal laser scan microscope. To detect the GSH levels we used a fluorescent MCB probe with excitation and emission wavelengths of 405 nm and 410-480 nm, respectively. The cell-permeating MCB probe is non-fluorescent but forms a fluorescent adduct with GSH in a reaction catalyzed by glutathione-S-transferase.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
入排标准
- 年龄范围
- 20 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •dry eye patients
- •control volunteers
排除标准
- •Young (less than 20) patients
- •The patients who rejected this study
结局指标
主要结局
Tear amount, tear break up time, and so on are measured clinically. The thiol redox status is measured by staining the membrane.
时间窗: Once a month (totally 2-4 times) at the clinic
次要结局
未报告次要终点