Lipolytic Effects of GH in Human Subjects in Vivo: Molecular Mechanisms and Temporal Patterns
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 9
- 试验地点
- 1
- 主要终点
- Lipolytic activity measured as area under the curve (AUC) for FFA (blood sample).
研究概览
简要总结
Growth hormone (GH) induces fat metabolism. The mechanisms underlying the fat metabolizing effects of GH remain elusive. However, it is known that insulin suppresses fat metabolism, and GH inhibits the expression of certain insulin-dependent signaling proteins. We therefore hypothesize that the fat metabolizing effects of GH depend on abrogation of insulin-dependent signaling pathways.
In order to investigate the fat metabolizing effects of GH, we'll analyze consecutive adipose tissue biopsies taken after GH exposure and GH blocking, respectively.
Knowledge of the effects of growth hormone and fat metabolism can in shot-sight as well as in long-sight have great importance for the understanding of growth disorders from overweight and type 2 diabetes to malnutrition and eating disorders.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy males
- •no medication
- •BMI 28-35
排除标准
- •known diseases
- •regular medication
研究组 & 干预措施
Growth hormone
Subjects are receiving growth hormone
干预措施: Growth Hormone (Drug)
Placebo
Subjects are receiving pegvisomant to block the action of growth hormone
干预措施: Pegisomant (Drug)
结局指标
主要结局
Lipolytic activity measured as area under the curve (AUC) for FFA (blood sample).
时间窗: 1 year
次要结局
- Substrate metabolism as measured by indirect calorimetry(3 months)
- GH signaling proteins in adipose tissue measured by western blotting(1,5 years)
- Intracellular lipolytic activity measured by an ATGL assay(1 year)
- GH signaling gene targets in adipose tissue measured by qPCR(1,5 years)