Impact of Training on Skeletal Muscle Vascular and Metabolic Changes With Limb Immobilization
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 试验地点
- 1
- 主要终点
- Angiogenesis
研究概览
简要总结
The study includes healthy young habitually active subjects. All subjects are subjected to two weeks of immobilization of one leg followed by four weeks of training of both legs. The subjects are randomized to either one-leg training of the non-immobilized leg during the two-week immobilization period or to no training of the non-immobilized leg during the immobilization period. Measures include skeletal muscle morphology, markers of angiogenesis and apoptosis, muscle metabolic markers, vascular function, vascular proteins and performance.
详细描述
One leg of young healthy subjects will be immoblized for two weeks. Changes in angiogenic proteins and markers of apoptosis in muscle tissue and muscle extracellular fluid will be determined in both legs and in parallel the functional consequences of immobilization will be assessed. Vascular function and vascular proteins will also be determined. In order to understand how the oxidative capacity of the muscle is altered in parallel with changes in capillarization, markers of mitochondria will be measured .Half of the subjects will be randomized to single-leg training during the immobilization period to assess whether simultaneous training reduces the loss of capillaries, muscle function and vascular function in the immobilized leg. After the immobilization period the study participants will train the immobilized leg and the control leg for four weeks by moderate to intense interval cycle training and the same parameteres as during immobilization will be assessed in both legs. The study will also include measurements of circulating compounds and platelets in blood.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Habitually/moderately physically active
排除标准
- •Known chronic medical disorder
- •Excessive alcohol intake
- •Known genetic/familial predisposition for thrombosis
- •Hormonal birth control
- •High level strength or endurance training on a regular basis
结局指标
主要结局
Angiogenesis
时间窗: Change from after immobilization to after 4 weeks of training
Skeletal muscle capillarization-comparison between legs
Vascular function
时间窗: Change from after immobilization to after 4 weeks of training
Measurements of skeletal muscle blood flow by ultrasound doppler in response to infusion of vasodilators/constrictors-comparison between legs
次要结局
- Markers of bone metabolism(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Knee extensor performance(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Vascular and metabolic proteins(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Maximal oxygen uptake(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Body composition(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Markers of angiogenesis(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Arterial blood pressure(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Skin microcirculation(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
- Vascular function-non-invasive(Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training)
研究者
Ylva Hellsten
Professor
University of Copenhagen