Estimation of Salivary volatile organic compounds in hypertensive subjects with periodontitis using Gas chromatography- Mass Spectrometry

Saliva is non-invasive and easily accessible fluid. It consist of volatile and non-volatile organic compounds responsible for the breath malodour. Halitosis/Breath malodour is an unpleasant smell from oral cavity.This originates from volatile sulphur compounds especially hydrogen sulphide, methyl mercaptan. Proteolytic degradation of sulphur and non sulphur containing amino acids by oral microorganisms in saliva, leads to release of malodorous compounds. The volatile organic compounds such as 1-Tetradecanol, 1-Hexadecanol, Toluene, Butylated hydroxytoluene and Dotriacontane are the products detected in saliva, breath and tongue-coating samples which is responsible for oral malodor. These compounds which are responsible for oral malodour are not observed in published studies in hypertensive patients with periodontitis because of difficulty in identification and quantification leading to exhaustive laboratory procedures. The putative pathogens responsible for periodontal disease are considered to be the prime causative factors for production of VOC causing oral malodour. Considering that periodontitis and hypertension share common risk factors,  the confounding factors must be controlled when assessing the association of these outcomes. Given the lack of published studies that have investigated the quantity of oral malodourous compounds in hypertensive subjects with or without periodontitis,this study is undertaken to evaluate malodourous compounds hypertensive patients with periodontitis. The subjects for the study will be selected from OPD in the Department of Periodontology, Manav Rachna Dental College, Faridabad. Selected subjects will be divided into 3 groups Group A, Group B and Group C based on hypertension and periodontal status. Group A consisting of Pre-Hypertensive  subjects with periodontitis, Group B comprising of Stage I Hypertensive periodontitis subjects  and Group C with Stage II Hypertensive periodontitis subjects .  All groups will undergo non-surgical periodontal therapy, recording of clinical parameters and saliva collection at baseline and at 1month. 3ml of unstimulated saliva will be collected by suction method and will be transferred to Eppendorf tubes. It will be stored at -80 degree centigrade till analysis. The Volatile organic compounds will be detected in saliva through the Gas chromatography- Mass spectrometry  analysis.