The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- Muscle protein breakdown rate
研究概览
简要总结
The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •18-40 years of age
- •Body mass index between 18.5 and 30
排除标准
- •Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
- •Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension
- •Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism)
- •A personal or family history of epilepsy, seizures or schizophrenia
- •Any known disorders in muscle metabolism
- •Regular use of nutritional supplements
- •Allergy to lidocaine
- •Allergy to one or multiple amino acids
- •Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches.
- •Having received or ingested a stable isotope tracer in the past
研究组 & 干预措施
Postabsorptive
Saline infusion to mimic postabsorptive circulating amino acid concentrations
干预措施: Postabsorptive (Other)
Postabsorptive
Saline infusion to mimic postabsorptive circulating amino acid concentrations
干预措施: Immobilisation (Procedure)
Postprandial
Amino acid infusion to mimic postprandial circulating amino acid concentrations
干预措施: Postprandial (Other)
Postprandial
Amino acid infusion to mimic postprandial circulating amino acid concentrations
干预措施: Immobilisation (Procedure)
结局指标
主要结局
Muscle protein breakdown rate
时间窗: 1 hour
Muscle protein breakdown rate (FBR, measured in %/h) during 1 hour of saline or amino acid infusion
次要结局
- Muscle protein breakdown rate(3 hours)
- Muscle protein synthesis rate(3 hours)