Effect of Sleep Restriction on Metabolic Disturbances Caused by Overfeeding
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 9
- 试验地点
- 2
- 主要终点
- Accumulation of fat in the liver (measured by nuclear magnetic resonance ) and the tissue insulin sensitivity ( measured by a test meal ) in response to a sleep restriction in overfeeding condition .
研究概览
简要总结
The purpose of this study is to determine if sleep restriction provokes an increase in risk factors for metabolic diseases, such as elevation of ectopic fat and decreased sensitivity to insulin. We propose to evaluate the effect of sleep restriction in a model of metabolic challenge (overfeeding) caricaturing the conditions of modern societies everyday life .
详细描述
General hypothesis :
In overfeeding condition, the stress response generated by partial sleep restriction of six days will induce an accumulation of fat in the liver and a decreased insulin sensitivity of adipose tissue , liver and muscle, more pronounced that in overfeeding condition only, provoked by a deregulation of the nocturnal lipolysis .
Specific goals :
Purpose 1 : To measure the accumulation of fat in the liver (measured by nuclear magnetic resonance ) and the tissue insulin sensitivity ( measured by a test meal ) in response to a sleep restriction in overfeeding condition .
Hypothesis 1: In overfeeding condition (130 % of energy requirements ) , hepatic lipid accumulation will be higher after 6 days of sleep restriction (4 hours per night) compared to a control condition (8 hours of sleep per night ) . Decreased sensitivity to insulin in the liver, adipose tissue and the associated muscle will be associated at this ectopic fat accumulation.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Good general health
- •BMI 18.5-25 kg/m2
- •Blood pressure at rest less than 140/ 90 mmHg .
- •Physical activity level: sedentary or light (< 3 hours of sport per week )
- •Lack of sleep pathology ( including sleep apnea and insomnia )
- •Regular sleep-wake cycles
- •Average duration of sleep over 7h
- •Circadian Typology by Horne & Ostberg questionnaire ≥ 30 and ≤ 70
- •Score questionnaire Pittsburgh Sleep Quality Index ≤ 5
- •Screening polysomnography showing sleep efficiency ≥ 85 % and apnea- hypopnea index and periodic leg movements < 15 / h .
排除标准
- •Current smoking
- •Family history of diabetes
- •History of sleep disorders
- •Circadian Typology by Horne & Ostberg [ 16-30 ] or [ 70-86 ]
- •Score questionnaire Pittsburgh Sleep Quality Inde x> 5
- •Polysomnography indicating a sleep efficiency <85% and apnea-hypopnea index and periodic leg movements > 15 / h.
- •Consumption of more than 3 coffees a day
- •Drinking alcool (more than 10g /d)
- •Use of Drugs
- •Medication
- •Night work by shifts
- •Average duration of sleep less than 7 hours per night
- •Occupational Risks in sleep restriction conditions: e.g. professional drivers
- •Special Diet ( vegetarianism, veganism , etc. )
- •Change in weight of more than 3 kg in the last 3 months
- •Consumption of more than 3 dl sugar-sweetened beverages per day ( sodas, energy drinks , fruit juice , flavored milk sugar )
- •Contraindication for magnetic resonance imaging ( pacemaker , previous eye surgery, the presence of metal implant or foreign body, Claustrophobia, etc. )
- •For women: pregnancy (current or desired)
- •Hemoglobin level < 13.5g/dl for men and < 12.5g/dl for women
- •Ferritin level < 50ug / l
- •Blood donation in the last 8 weeks
研究组 & 干预措施
Overfeeding + sleep restriction
overfeeding condition (130 % of energy requirements ) + 6 days of sleep restriction (4 hours per night)
干预措施: Overfeeding (130 % of energy requirements ) (Dietary Supplement)
Overfeeding + sleep restriction
overfeeding condition (130 % of energy requirements ) + 6 days of sleep restriction (4 hours per night)
干预措施: Sleep restriction (4 hours per night) (Behavioral)
Overfeeding + normal sleep duration
overfeeding condition (130 % of energy requirements ) + 6 days of normal sleep duration (8 hours per night)
干预措施: Overfeeding (130 % of energy requirements ) (Dietary Supplement)
结局指标
主要结局
Accumulation of fat in the liver (measured by nuclear magnetic resonance ) and the tissue insulin sensitivity ( measured by a test meal ) in response to a sleep restriction in overfeeding condition .
时间窗: After 6 days of sleep restriction (4 h/day) vs. normal sleep (8 h/day)
次要结局
- Blood concentrations of circulating fatty acids and glycerol(After 6 days of sleep restriction (4 h/day) vs. normal sleep (8 h/day))
研究者
José HABA-RUBIO
MD
University of Lausanne Hospitals