Study of Pomegranate Juice in the Modulation of Oxidative Stress Markers in Endurance-based Athletes
试验速览
- 阶段
- 早期 1 期
- 状态
- 已完成
- 入组人数
- 20
- 主要终点
- Protein carbonyls levels changes in plasma
研究概览
简要总结
The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Perform specific endurance training sessions
- •More than 1 hour per session and more than 3 sessions per week
- •Have participated recently in a half marathon or similar event
排除标准
- •Intake of antioxidant or anti-inflammatory supplementation
- •Present a chronic disorder
- •Non-smoker
- •No-alcoholic beverage consumption
结局指标
主要结局
Protein carbonyls levels changes in plasma
时间窗: day 0 and 22
Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein
Malondialdehyde levels changes in plasma
时间窗: day 0 and 22
Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein
次要结局
- Blood biochemical parameters changes in plasma(day 0 and 22)
- C-reactive protein levels changes in plasma(day 0 and 22)
- Soluble E-selectin levels changes in plasma(day 0 and 22)
研究者
Néstor Vicente-Salar
PhD
Universidad Miguel Hernandez de Elche