Relationship Between Micro Ribonucleic Acid (miRNA) Expression and Inflammatory Factors in Patients With Parkinson's and Periodontal Disease
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 51
- 试验地点
- 2
- 主要终点
- 1. Pocket probing depth
研究概览
简要总结
MicroRNAs are important regulators of the immune and inflammatory response to bacterial pathogens during periodontal disease.The aim of this study was to evaluate the possible miRNA type for Parkinson's in saliva of orally healthy individuals.
详细描述
A total of 51 individuals, 35 systemically healthy and 16 with Parkinson's disease and both dental and periodontal health, were included in the study.Clinical periodontal parameters, DMFT data and salivary flow rates were recorded.Unstimulated saliva samples were taken from each participant and miRNA was evaluated by RT-qPCR method.ELISA method was used to measure TNF α values in collected saliva and serum samples.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •dentally and periodontally healthy
- •Systemically healthy with no uncontrolled chronic and/or autoimmune disease
- •No systemic, neurological, or autoimmune disease in addition to Parkinson's disease.
排除标准
- •history of regular use of systemic antibiotics anti-inflammatory, or antioxidant drugs (previous 3 months)
- •nonsurgical periodontal treatment (previous 6 months)
- •surgical periodontal treatment (previous 12 months)
- •presence of<20 teeth
- •current medications affecting gingival health (calcium channel blockers, phenytoin, cyclosporine, and hormone replacement therapy)
- •diagnosis of rheumatoid arthritis
- •pregnancy
- •lactating
- •excessive alcohol consumption.
研究组 & 干预措施
Active Comparator
Collection of patients' saliva and serum and analysis of sample molecules.
干预措施: Saliva and Serum (Other)
Experimental
ELISA method will be used to measure TNF-α values in collected saliva and serum samples.RT-qPCR based miRNA gene expression analyses will be performed with 2-step qRT-PCR Kits (Thermofisher Scientific, MA, USA).
干预措施: Saliva and Serum (Other)
结局指标
主要结局
1. Pocket probing depth
时间窗: 6 months
Measurement of the depth of a sulcus or periodontal pocket determined by measuring distance from a gingival margin to the base of the sulcus or pocket with a calibrated periodontal probe.
1. Pocket probing depth
时间窗: 6 months
Measurement of the depth of a sulcus or periodontal pocket determined by measuring distance from a gingival margin to the base of the sulcus or pocket with a calibrated periodontal probe.
2.Clinical attachment level
时间窗: 6 months
Clinical attachment level (or loss, CAL) is a more accurate indicator of the periodontal support around a tooth than probing depth alone. CAL is measured from a fixed point on the tooth that does not change, the CEJ. To calculate CAL, two measurements are needed: distance from the gingival margin to the CEJ and probing depth. In recession: probing depth (+) gingival margin to the CEJ (add). In tissue overgrowth: probing depth (-) gingival margin to the CEJ (subtract)
3.Bleeding on probing
时间窗: 6 months
referring to bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus, or interface between the gingiva and a tooth.
次要结局
- 4.Saliva and serum samples processing and analyses(1 month)
- 4.Saliva and serum samples processing and analyses(1 month)
研究者
Melis Yilmaz
Assistant Prof Dr
Medipol University