Salivary Gasdermin E and Granzyme B Levels in Patients With Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 46
- 试验地点
- 1
- 主要终点
- Salivary Gasdermin E Concentration
研究概览
简要总结
This observational study aims to compare salivary Gasdermin E and Granzyme B levels between individuals with periodontitis and periodontally healthy individuals. It will also evaluate the relationships between these biomarkers and clinical periodontal measurements. Saliva samples and periodontal clinical measurements will be collected from all participants. No treatment or experimental intervention will be administered as part of the study. The findings may contribute to a better understanding of the inflammatory mechanisms associated with periodontitis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Individuals aged 18 to 65 years
- •Presence of at least 20 natural teeth, excluding third molars
- •Being systemically healthy
- •Provision of written informed consent
- •For the periodontitis group, diagnosis of generalized Stage III Grade B periodontitis according to the 2017 AAP/EFP classification
- •Clinical attachment loss of at least 2 mm at two or more non-adjacent teeth for the periodontitis group
- •Interdental clinical attachment loss of at least 5 mm and periodontitis-related loss of no more than four teeth for Stage III periodontitis
- •A radiographic bone loss-to-age ratio between 0.25 and 1.00 for Grade B periodontitis
- •For the healthy control group, an intact periodontium, probing depth of 3 mm or less, and bleeding on probing at less than 10% of sites
排除标准
- •Use of antibiotics, antimicrobial agents, nonsteroidal anti-inflammatory drugs, steroids, immunosuppressants, beta-blockers, calcium channel blockers, anticoagulants, or hormonal contraceptives within the previous 3 months
- •Periodontal treatment within the previous 6 months
- •Surgical periodontal treatment within the previous 12 months
- •Current smoking
- •Pregnancy or breastfeeding
- •Presence of any neurological, autoimmune, metabolic, inflammatory, or other systemic condition that could affect the immune response
- •Use of an orthodontic appliance
结局指标
主要结局
Salivary Gasdermin E Concentration
时间窗: At baseline (single study visit)
Gasdermin E concentration in unstimulated whole saliva samples will be measured using an enzyme-linked immunosorbent assay (ELISA) and compared between the periodontitis and periodontally healthy groups.
Salivary Granzyme B Concentration
时间窗: At baseline (single study visit)
Granzyme B concentration in unstimulated whole saliva samples will be measured using an enzyme-linked immunosorbent assay (ELISA) and compared between the periodontitis and periodontally healthy groups.
次要结局
- Correlation Between Salivary Gasdermin E Levels and Clinical Periodontal Parameters(At baseline (single study visit))
- Correlation Between Salivary Granzyme B Levels and Clinical Periodontal Parameters(At baseline (single study visit))
研究者
Merve Can
Assistant Prof Dr.
Medipol University