Assessment of the Expression and Activity of Protein Kinase A in Prostate Cancer Tissue
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 100
- 主要终点
- The distribution of PKA in prostate cancer versus benign tissue
研究概览
简要总结
Prostate cancer is the most common malignancy in men. documented risk factors of prostate cancer are age, ethnicity, various genomic mutations and family history of prostate cancer. The cellular mechanisms of malignant transformation are numerous and not completely understood. A possible mechanism is induction of an inflammation resulting in cellular atypia and pre-malignant changes in the affected tissue by inducing a pro-inflammatory response or changes in extra cellular matrix. Protein Kinase A (PKA) is a key stone enzyme in various intra-cellular processes. Various infections' inflammations and malignancies were proved to impact PKA activity. The research hypothesis is that prostate cancer tissue will show a unique profile of PKA activity, regulation and intracellular distribution.
详细描述
The study will be conducted among men referred to prostate biopsy due to clinical suspicion of malignancy. 100 consecutive patients will undergo prostate biopsies. Biopsies will be taken from both lobes of the prostate (standard protocol) and additional biopsies will be taken according to MRI findings (Fusion biopsies) if relevant. One additional biopsy will be taken from each lobe of the prostate and immediately freezed in -80 Celsius degrees. Routine biopsies will be fixed in formaldehyde and embedded in paraffin and will undergo routine pathological analysis and staining.
Following pathological analysis, paraffin slides will be undergo immunohistochemically staining for PKA, comparing cancerous tissue to normal surrounding tissue. the fresh frozen tissue will undergo western blot analysis for quantitative expression of PKA and its sub units.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 50 Years 至 —（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Clinical suspicion of prostate cancer
- •Referred for biopsy
- •Able to sign informed consent.
排除标准
- •Previous prostate irradiation
- •Chronic prostatitis
- •Previous prostate surgery
- •Recent urinary tract infection (3 months prior to biopsy).
- •Indwelling urinary catheter
结局指标
主要结局
The distribution of PKA in prostate cancer versus benign tissue
时间窗: 1 year
Immunohistochemical staining of paraffin sections of prostatebiopsies for PKA, western blot analysisi of tissue.
次要结局
未报告次要终点
研究者
Ran Katz
Dr. Ran Katz, Head, department of Urology, ziv medical center
Ziv Hospital