The Impact of Dietary Intervention Using Innovative Food with High Antioxidant Potential on Oxidative/antioxidant Markers in Blood and Gut Microbiota in Athletes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Change in gut microbiota after dietary intervention
研究概览
简要总结
During intense physical exercise, there is an overproduction of reactive oxygen species, which leads to oxidative stress and reduced training and sports performance, as well as the development of chronic diseases. Eating foods with a high content of bioactive ingredients and high antioxidant potential can alleviate the negative effects caused by reactive oxygen species and improve the state of intestinal microflora.
The aim of these interventional studies was to determine whether daily consumption of foods with high antioxidant potential, including fruit and nut bars, for a period of 1 month would reduce oxidative stress in athletes during competition and positively change the intestinal microflora.
详细描述
The study concerns a 4-week dietary intervention. The intervention consisted of daily consumption of 1 bar weighing 50 g, containing nuts and dried fruits with high antioxidant potential. The study included a group of 50 women and men, healthy athletes (cyclists, long-distance runners and triathletes). A 3-day, 24-hour dietary interview was conducted before and after the dietary intervention. Then, using the Dieta 6.0 computer program, the energy and nutrient contents in the diet were estimated. In addition, adherence to the dietary intervention was assessed using a survey questionnaire. Before and after the dietary intervention, oxidative-antioxidant markers were determined in the blood of the subjects (oxidative stress, antioxidant potential, concentration of antioxidant enzymes: glutathione peroxidase, superoxide dismutase, catalase) using ready-made kits. Moreover, changes in the intestinal microbiome were assessed in feces before and after the dietary intervention. Additionally, height and weight were measured before and after the intervention to calculate body mass index (BMI). The study will help answer the question of whether dietary intervention using foods with high antioxidant potential can influence changes in oxidative-antioxidant markers in the blood, intestinal microbiome and body weight of study participants.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •healthy men and women with high physical activity (triathletes, cyclists, long-distance runners), not taking medications for chronic diseases, age 20-50 years, men and women.
排除标准
- •occurence of chronic diseases (e.g. hypertension, diabetes, cancer, thyroid diseases, neurodegenerative diseases), taking medications for chronic diseases, age<20 and >50 years, low physical activity
研究组 & 干预措施
Oxidative-antioxidant markers in blood and gut microbiota
Before and after the 1-month dietary intervention, the following markers will be determined in the blood: antioxidant potential, antioxidant enzymes, oxidative stress. Moreover, the intestinal microbiota will be determined before and after the intervention.
干预措施: Food with high antioxidant potential (Other)
结局指标
主要结局
Change in gut microbiota after dietary intervention
时间窗: 4 weeks
The gut microbiota in feces was determined before and after the dietary intervention in a hospital laboratory.
Change in antioxidant potential after dietary intervention
时间窗: 4 weeks
Before and after the dietary intervention, the antioxidant potential in the blood was determined using the ABTS method.
Assessment of dietary intervention
时间窗: 4 weeks
The dietary intervention lasted 4 weeks. The subjects received one bar a day weighing 50 g, containing nuts and dried fruits with high antioxidant potential and high polyphenol content. After the dietary intervention, the participants completed a survey containing questions about compliance with the recommendations of the dietary intervention.
Change in blood glutathione peroxidase concentration after dietary intervention
时间窗: 4 weeks
Before and after the dietary intervention, the concentration of glutathione peroxidase enzyme in blood was assessed using ready-made laboratory kits.
Change in superoxide dismutase concentration after dietary intervention
时间窗: 4 weeks
Before and after the dietary intervention, the concentration of superoxide dismutase enzyme in blood was assessed using ready-made laboratory kits.
Change in catalase concentrations after dietary intervention
时间窗: 4 weeks
Before and after the dietary intervention, the concentration of catalase enzyme in blood was assessed using ready-made laboratory kits.
Change in oxidative stress after dietary intervention
时间窗: 4 weeks
Before and after the dietary intervention, the oxidative stress in blood was assessed using ready-made laboratory kits.
次要结局
- Assessment of dietary nutrients before and after dietary intervention(4 weeks)
- Body weight assessment before and after dietary intervention(4 weeks)
- Measuring the height of study participants(4 weeks)