Regulation of S100A10 by the PML-RAR-alpha Oncoprotein
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 主要终点
- Demonstration that S100A10 is expressed on acute promyelocytic leukemia cells and that levels of S100A10 correspond to the amount of fibrinolytic activity
研究概览
简要总结
RATIONALE: Studying samples fo tissue from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer.
PURPOSE: This research study is studying biomarkers in tissue samples from patients with acute promyelocytic leukemia.
详细描述
OBJECTIVES:
- Demonstrate that S100A10 is expressed on APL cells and that levels of S100A10 correspond to the amount of fibrinolytic activity.
- Evaluate the impact of ATRA on S100A10 level and fibrinolytic activity in vitro.
- Evaluate the impact of S100A10 knockdown on fibrinolytic activity.
- Correlate in vitro findings in cell culture to primary patient samples both pre- and post-ATRA therapy.
OUTLINE: This is a multicenter study.
Previously collected samples are analyzed via flow cytometry and western blot analysis. Cell surface levels of S100A10 and annexin A2 are correlated to the promyelocyte population and total protein levels are examined to determine total annexin A2 and S100A10 in acute promyelocytic leukemia cells.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- — 至 120 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Demonstration that S100A10 is expressed on acute promyelocytic leukemia cells and that levels of S100A10 correspond to the amount of fibrinolytic activity
Evaluation of the impact of all-trans retinoic acid (ATRA) on S100A10 level and fibrinolytic activity in vitro
Evaluation of the impact of S100A10 knockdown on fibrinolytic activity
Correlation of in vitro findings in cell culture to primary patient samples both pre- and post-ATRA therapy
次要结局
未报告次要终点