Effect of Repeated Brief Passive Heat Therapy on Metabolism in Healthy Young Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 25
- 试验地点
- 1
- 主要终点
- Changes in cytokines concentration (pg/mL)
研究概览
简要总结
The goal of this prospective interventional study is to examine if repeated brief hot stimuli affects glucose metabolism and substrate oxidation in young non-obese adults. Young adult participants were asked to participate in fourteen 5-min procedures involving whole body passive heating at 45°C water.
The main question it aims to answer is: "Does repeated brief noxious heat stimuli is sufficient to improve glucose tolerance, insulin sensitivity, and fat oxidation in young non-obese adults?"
详细描述
No studies yet addressed whether brief heat stimuli could be viable time-efficient alternative approach in order to improve glucose metabolism and fat oxidation. Consequently, we aimed to examine the ability of brief noxious heat stimuli to improve glucose tolerance, insulin sensitivity, and fat oxidation in young adults. Non-obese males and females completed fourteen 5-min sessions involving whole body passive heating at 45°C water. Changes in catecholamines, cytokines, substrate oxidation, resting energy expenditure, glucose tolerance and insulin response were assessed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •healthy non-obese (BMI between 18.5 and 29.9 kg/m2) males and females;
- •no diseases, or conditions that could be worsened by exposure to acute hot water and affect experimental variables;
- •no participation in any excessive formal physical exercise or sports program, temperature manipulation program or exposure to extreme temperatures.
排除标准
- •obesity (BMI greater than 30 kg/m2);
- •needle phobia;
- •taking medication and/or dietary supplements that may affect experimental variables.
结局指标
主要结局
Changes in cytokines concentration (pg/mL)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
The venous serum interleukin-6 and tumor necrosis factor alpha concentrations (in pg/mL) were measured using enzyme-linked immunosorbent assay kits and a Spark multimode microplate reader
Change in resting energy expenditure (kcal/day)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the resting energy expenditure (REE; kcal/day) was calculated by using the Weir equation: REE = (3.941(VO2) + 1.106(VCO2)) × 1440.
Change in glucose concentration (mmol/L)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
The venous glucose concentration (in mmol/L) was measured using a Glucocard X-mini plus meter.
Changes in catecholamines concentration (ng/mL)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
The venous plasma adrenaline and noradrenaline concentrations (in ng/mL) were measured using enzyme-linked immunosorbent assay kits and a Spark multimode microplate reader
Change in insulin sensitivity
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
Indices for insulin sensitivity/resistance assessment were computed using the homeostatic model assessment for insulin resistance, quantitative insulin-sensitivity check index (QUICKI), and the Matsuda insulin sensitivity index were calculated.
Change in fat oxidation (g/min)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the carbohydrate oxidation (CARBox; g/min) was calculated by using the equation: CARBox = 4.55 × VCO2 - 3.21 × VO2
Change in insulin concentration (μIU/mL)
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
The venous serum insulin concentrations (in μIU/mL) were measured using enzyme-linked immunosorbent assay kits (Cat. No. E-EL-H2237, Elabscience, China) and a Spark multimode microplate reader (Tecan, Austria).
Change in substrate oxidation
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the respiratory quotient (RQ=VCO2/VO2) was computed to determine substrate utilisation.
Change in fat oxidation (g/min
时间窗: Pre-condition, post-condition (after 14 days), and after 1 month recovery
Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the fat oxidation (FATox; g/min) was calculated by using the equation: FATox = 1.67 × VO2 - 1.67 × VCO2,
次要结局
- Change in fat mass (kg)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in body mass (kg)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in fat free mass (kg)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in body mass index (kg/m2)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in oxygen consumption and carbon dioxide output (mL/min)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in fat free mass (%)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)
- Change in fat mass (%)(Pre-condition, post-condition (after 14 days), and after 1 month recovery)