Gene Expression Profiling of Metastatic Colon Cancer (CCCWFU 89B03)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 主要终点
- Gene expression changes that occur at each metastatic site (i.e., liver, lungs, and peritoneum)
研究概览
简要总结
RATIONALE: Studying the genes expressed in samples of tissue from patients with cancer may help doctors identify biomarkers related to cancer.
PURPOSE: This laboratory study is using gene expression profiling to evaluate normal tissue and tumor tissue from patients with colon cancer that has spread to the liver, lungs, or peritoneum.
详细描述
OBJECTIVES:
- Evaluate gene expression profiles in normal and tumor tissue from patients with colon cancer metastatic to the liver, lungs, or peritoneum.
- Establish cell lines from primary colon tumors metastatic to the liver, lungs, or peritoneum.
- Determine the specific gene expression changes that result in the manifestation of the drug-resistant phenotype for each metastatic site.
OUTLINE: This is a pilot study.
Tumor and normal tissue collected during surgery are analyzed for gene expression profiling by cDNA microarray. Tissue is also analyzed for thymidylate synthase (TS) gene expression by quantitative PCR and for protein expression by western blot. Gene expression patterns are correlated with TS levels.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- — 至 120 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Gene expression changes that occur at each metastatic site (i.e., liver, lungs, and peritoneum)
时间窗: day 1
Development of cell lines from primary colon tumors metastatic to the liver, lungs, or peritoneum
时间窗: day 1
次要结局
未报告次要终点