Damage to DNA Caused by UV-A Irradiation: Photochemical Mechanism and Cutaneous Parameters Involved in the Formation of Cyclobutane Pyrimidine Dimers
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 24
- 试验地点
- 4
- 主要终点
- Phototype determination according to the Fitzpatrick classification Number of CPD and oxidative lesions determinated by the analysis of DNA from the skin biopsies after their ex-vivo exposure to UV-A - The CPD / Oxidative lesions ratio
研究概览
简要总结
The DIMUVA study aims to evaluate the correlation between cutaneous phototype and the nature and quantity of damage caused to cutaneous DNA after exposure to UV-A radiation.
详细描述
Due to their capacity to damage Deoxyribonucleic acid (DNA), Ultra-Violet (UV) radiation is one of the causes of skin cancers.
Until recently, the genotoxic effects of UV-A radiation, were poorly identified, in particular their capacity to lead to the dimerization of pyrimidine bases .
It is well known that the response to UV-A and UV-B radiations is different depending on the cutaneous phototype.
Thus, the aim of this study is to determine the correlation between cutaneous phototype and the quantity and nature (CPD or oxidative lesions) of damage caused to cutaneous DNA after an ex-vivo exposure to UV-A and UV-B radiations.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Health Services Research
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Between 18 and 35 years old,
- •Healthy volunteers,
- •Cutaneous phototype 2 or 4 according to the Fitzpatrick classification,
- •Affiliation to the French Social Security.
排除标准
- •History of photosensibility,
- •Active smoking or stopped since less than one year,
- •Dermatological pathology or treatment contra-indicating cutaneous irradiation and skin biopsies,
- •Any chronic pathology susceptible to interfere with the evaluations related to the protocol,
- •Allergy to local anaesthetics,
- •Volunteers who take drugs and/or food complements acting on oxidative stress in the 8 weeks preceding inclusion,
- •Volunteers who have take paracetamol or aspirin within 7 days prior to the inclusion visit,
- •Subject in exclusion period for another biomedical research study,
- •Subject having exceeded the threshold of annual compensation for biomedical research.
结局指标
主要结局
Phototype determination according to the Fitzpatrick classification Number of CPD and oxidative lesions determinated by the analysis of DNA from the skin biopsies after their ex-vivo exposure to UV-A - The CPD / Oxidative lesions ratio
时间窗: Day 0
次要结局
- Number of CPD and oxidative lesions determinated by the analysis of DNA from the skin biopsies after their exposure ex-vivo to UV-B.(Day 0)
- UV-A radiation exposure: Minimal erythemic dose - Number of CPD and oxidative lesions - CPD / oxidative lesions ratio(Day x)
- UV-B radiation exposure: Minimal erythemic dose - Number of CPD and oxidative lesions - CPD/oxidative lesions ratio(Day 0)
- antioxidant status and quantity of CPD, oxidative lesions after exposure to UV-A and UV-B radiations(day 2)