Effect of Food Acceptability on Appetite Hormones' Response in Normal Weight vs. Obese Male Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 30
- 主要终点
- GLP-1
研究概览
简要总结
Male subjects with specific body characteristics were recruited for the study. Subjects did an acceptability test on custard and completed a screening questionnaire. Around 30 subjects (15 with a normal BMI and 15 obese) continued the study. At each test day, fasting blood samples were withdrawn by a registered nurse under sterile conditions. This procedure was followed by ingestion of one of the two meals within a period of 10-15 minutes. Blood samples of 5 ml were collected at fasting and after ingestion of meals. At the end, subjects were given ad lib access to cheese pizza to assess energy intake after the custard preload. Blood analysis for GLP-1, ghrelin, insulin and glucose levels were performed.
详细描述
Male subjects were recruited for the study based on the following criteria: age between 18 and 50, body mass index normal or obese, non-smokers, non-alcoholic, and do not take medications. Subjects did an acceptability test on custard and completed a screening questionnaire. Around 30 subjects (15 with a normal BMI and 15 obese) continued the study. Subjects were randomly assigned to one of two meals. Each of the two meals was ingested after a 3-day adaptation period. The meals had similar macronutrient composition and provided energy equivalent to 30% of the subjects' resting energy expenditure. At each test day, fasting blood samples were withdrawn by a registered nurse under sterile conditions. This procedure was followed by ingestion of one of the two meals within a period of 10-15 minutes. Blood samples of 5 ml were collected at fasting and after ingestion of meals at 15, 30, 60, 120, 180 and 240 minutes. At the end, subjects were given ad lib access to cheese pizza to assess energy intake after the custard preload. Blood analysis for GLP-1, ghrelin, insulin and glucose levels were performed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Gender: Male
- •Age: 18-50 years
- •Body Mass Index (BMI): 18.5-24.9 kg/m2 or 30-39.9 kg/m2
- •Stable body weight for at least three months before the study with the absence of any form of dieting, food restriction or other abnormal eating behaviors (to minimize the effect of weight change on ghrelin and GLP-1 statuses)
- •Agreement on the acceptability levels of the two versions (high and low acceptability) of custard
排除标准
- •Substance abuse such as alcohol or drugs
- •Medical or psychological illness
- •Previous gastrointestinal surgery
- •History of weight fluctuation (weight loss of greater than 5% within the past 3 months)
研究组 & 干预措施
Lean panelists
Lean panelists or normal weight panelists with a BMI between 18.5 and 24.9 Acceptability was assessed
干预措施: Acceptability (Other)
Obese panelists
Obese panelists with a BMI between 25 and 29.9 Acceptability was assessed
干预措施: Acceptability (Other)
结局指标
主要结局
GLP-1
时间窗: Through study completion, an average of 1 year
GLP-1 levels measured with Enzyme-linked immunosorbent assay (ELISA) kit
Insulin
时间窗: Through study completion, an average of 1 year
Insulin levels measured with Enzyme-linked immunosorbent assay (ELISA) kit
Food acceptability
时间窗: Through study completion, an average of 1 year
Food acceptability on a 9 point hedonic scale
Ghrelin
时间窗: Through study completion, an average of 1 year
Ghrelin levels measured with Enzyme-linked immunosorbent assay (ELISA) kit
Glucose
时间窗: Through study completion, an average of 1 year
Glucose levels measured using VITROS ECiQ Immunodiagnostic System
次要结局
- Appetite(Through study completion, an average of 1 year)
- Energy intake at the next meal(Through study completion, an average of 1 year)
研究者
Ammar Olabi
Professor
American University of Beirut Medical Center