CpG methylation is a relevant epigentic modification in sepsis

Not Applicable

• Conditions: healthy subjects

• Registration Number: DRKS00012965
• Lead Sponsor: niversitätsklinikum Knappschaftskrankenhaus Bochum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2018-03-23
• Study Completion: 2019-04-03
• Results First Posted: 2020-10-13
• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Complete
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

voluntary, healthy individuals age >18

Exclusion Criteria

low body weight, too youn age <18 and too old age>65, anemia

Study & Design

• Study Type: interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Evaluation of a modified mitochondrial translation of the DNMT1 before LPS stimulation and after LPS stimulation during the time course (after ½, 2/4/8/24/48 hours) by Wester blot and immunofluorescence staining.

Secondary Outcome Measures

Name	Time	Method
Evaluation of a modified methylation pattern on the cytosine of the mitochondrial DNA before LPS stimulation and after LPS stimulation during the time course (after ½, 2/4/8/24/48 hours) by immunofluorescence staining and genetic methylation analysis. In addition, a possible association between inflammation and anti-inflammation and epigenetic changes in the mtDNA is to be investigated.