Role of Adipose Tissue in Pathogenesis of Insulin Resistance
- Conditions
- ObesityInsulin Resistance
- Registration Number
- NCT00439491
- Lead Sponsor
- Charles University, Czech Republic
- Brief Summary
The aim of the study was to investigate the relationship between insulin resistance and body composition in relation to secterion and expression of adipocytokines. Obese subjects undergo 6 months hypocaloric diet and, before, during and at the end of the diet, plasma samples and subcutaneous adipose tissue samples are obtained for subsequent analysis. In addition, body composition and insulin resistance are measured before and during the diet.
- Detailed Description
The aim of the study was to investigate the relationship between insulin resistance and body composition in relation to secterion and expression of adipocytokines. Obese subjects undergo 6 months hypocaloric diet without any medication and, before, during and at the end of the diet, plasma samples and subcutaneous adipose tissue samples are obtained for subsequent analysis. Adipose tissue samples are obtained using needle biopsy. In addition, before and during the diet,body composition is measured using multifrequency bioimpedance and insulin resistance is evaluated using either hyperinsulinemic clamp or glucose/insulin - derived indices.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 35
- Obesity
- Diabetes
- Coronary heart disease
- Eating disorders
- Medication of beta blockers
- Medication of antiobesity drugs
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Primary Outcome Measures
Name Time Method Body weight Body fat
- Secondary Outcome Measures
Name Time Method Insulin resistance Adipocytokine plasma levels Adipocytokine adipose tissue expression
Trial Locations
- Locations (1)
Third Faculty of Medicine, Charles University in Prague
🇨🇿Prague, Czech Republic