Detecting the antigen-specific B-cell-response to rabies vaccination.
- Conditions
- Rabies
- Registration Number
- NL-OMON24167
- Lead Sponsor
- MC
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 20
In order to be eligible to participate in this study, a hyperimmunized subject must meet all of the following criteria:
- 18 years old or older.
- Received at least 3 rabies vaccinations.
- Able to provide informed consent.
A control subject must meet the following criteria:
- 18 years old or older.
- Never received rabies vaccinations.
- Able to provide informed consent.
A potential subject who meets any of the following criteria will be excluded from participation in this study:
- History of (pre)syncope associated with medical procedures involving needles.
- Received any vaccination other than rabies three months prior to inclusion.
- Administration of plasma or blood products three months prior to inclusion.
- Bleeding disorders or use of anticoagulants.
- Any current infectious disease other than seasonal cold.
- Immunocompromised (due to medication, medical condition, or other).
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method An optimal combination of concentrations of rabies virus and rabies-specific antibodies will be used to detect and quantify the population of rabies-specific B-cells using flow cytometry. We expect to see no rabies-positive B-cell events in unvaccinated individuals. If we see a rabies-positive B-cell population in hyperimmunized individuals, we can conclude that our assay is able to detect rabies-specific B-cells, which is our primary endpoint.<br>This outcome will be measured in blood drawn from the participants via venipuncture at one single timepoint. This timepoint is independent of the most recent rabies vaccination.
- Secondary Outcome Measures
Name Time Method In an explorative setting, we want to see if we can quantify the population of rabies-specific B-cells in different donors, using flow cytometry. Furthermore, we would like to explore if we can further classify the rabies-specific population into different B-cell subsets.This outcome will be measured in blood drawn from the participants via venipuncture at one single timepoint. This timepoint is independent of the most recent rabies vaccination.