The Role of TLR-4 Asp299Gly and Thr399Ile Polymorphisms in Children With Urinary Tack Infections
试验速览
- 阶段
- 不适用
- 入组人数
- 160
- 试验地点
- 1
- 主要终点
- frequency of TLR-4 299, 399 polymorphism and expression of TLR-2 and TLR-4 by monocytes in children with urinary track infection and healthy controls.
研究概览
简要总结
Purpose So far anatomical abnormalities (mostly congenital) were, in the majority of the patients, associated with urinary track infections. In this study the researchers will try to investigate the role of TLRs as molecular interactions between bacterial virulence and host response.
TLRs are important mediators in the development of the natural immunity against bacteria. They recognize microbial pathogen associated molecular patterns and alert the host's immune system to the presence of invading microbes
详细描述
The aim of his observational case-control study is to :
- investigate if the expression of TLR2 and TLR4 mediate in the development of the natural immunity of the uroepithileal cells against bacteria.
- evaluate the effect of the TLR-gene Asp299Gly and Thr399Ile polymorphisms on the development and clinical severity of urinary tract infections (UTI) in children.
- determine the association of the expression of TLR-2 and TLR-4 and TLR-4 polymorphisms with the formation of renal scar in children.
- investigate the expression of TLR-2 and TLR-4 and TLR-4 polymorphisms in healthy children and elaborate the results to the possible carriers of these polymorphisms.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 6 Months 至 14 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •For the patients
- •6months -14 years of age
- •unrelated male and female children with one episode of acute urinary track infection (positive urine culture and clinical symptoms )
- •with/without anatomic genitourinary anomalies .
- •children with upper UTI or children with lower UTI
- •Sign written informed consent
- •Inclusion Criteria:
- •For the controls
- •6 months-14 years of age
- •age and gender matched with the patients
- •unrelated male and female children with no recorded history of urinary track infection and negative urine cultures .
- •Sign written informed consent
排除标准
- •For the patients
- •Subjects who do not meet the criteria above
- •Severe or chronic illness
- •Symptoms of acute infection
- •Immune deficiency or immune suppressor therapy
- •Medication
- •Exclusion Criteria:
- •For the controls
- •Subjects who do not meet the criteria above
- •Severe or chronic illness
- •Symptoms of acute infection
- •Immune deficiency or immune suppressor therapy
- •Medication
结局指标
主要结局
frequency of TLR-4 299, 399 polymorphism and expression of TLR-2 and TLR-4 by monocytes in children with urinary track infection and healthy controls.
时间窗: 4 years
次要结局
未报告次要终点
研究者
Panagiota Karananou
MD
Aristotle University Of Thessaloniki