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Effect of White Potatoes on Glycemic Response, Satiety and Food Intake in Children

Not Applicable
Completed
Conditions
Healthy
Interventions
Other: Baked potato with skin
Other: Meal skipping
Other: White bread
Other: Mashed potato served hot
Other: Fried French fries
Registration Number
NCT03307681
Lead Sponsor
Toronto Metropolitan University
Brief Summary

The purpose of the present study is to explore the influence of cooking methods on post-prandial glycaemia and its impact on satiety in children.

Detailed Description

Each of the 5 study sessions will be at least 7 days apart. Either meal skipping, or one of three treatments of white potatoes (a) baked (with skin), (b) mashed, (c) fried French fries, or white bread, prepared on the day of testing, will be served to healthy children (9-14 years). Participants will consume the equivalent to 1 medium sized potato (\~280 kcal) or an equivalent amount of calories from white bread. Glycemic response, insulin, incretin hormones (glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic peptide (GIP)), dipeptidyl peptidase 4 (DPP4), and cholecystokinin (CCK) will be measured for 2 h (0, 15, 30, 45, 60, 90 and 120 min) following meal consumption, as well as mood and subjective appetite. An ad libitum test meal will be provided at 120 min to assess food intake suppression.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
14
Inclusion Criteria
  • be between 9 and 14 years of age
  • be healthy, and have been born at term
  • be a native English speaker
  • not be taking any medications
  • not have allergies to potatoes, rice, or beans.
  • normal body weight (between the 5th and 85th BMI percentile for age and gender)
Exclusion Criteria
  • anyone with food sensitivities or allergies to potatoes or potato-products,
  • smokers
  • diabetic or overweight/obese individuals.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Baked potato with skinBaked potato with skinBaked russet potato
Meal skippingMeal skippingNo food given
White breadWhite breadMatched for energy content and available carbohydrate content of potato treatments
Mashed potato served hotMashed potato served hotMashed potatoes prepared from frozen, matched for available carbohydrate content of baked potato
Fried French friesFried French friesMatched for available carbohydrate content of baked potato
Primary Outcome Measures
NameTimeMethod
Ad libitum food intake (lunch, at 120 minutes)at 120 minutes post morning meal consumption

Food intake will be determined by weighing the meal before and after serving. The net weight of the test meal will be converted to calories

Change from baseline glycemic response15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood glucose (mmol/L). Blood glucose will be measured in whole blood using YSI 2300 STAT PLUS (YSI Incorporated, Yellow Springs, OH)

Change from baseline insulin15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood insulin (pmol/L). Insulin concentration in serum will be determined in duplicate via enzyme-linked immunosorbent assay kits (ELISA; Millipore, Billerica, Massachusetts).

Secondary Outcome Measures
NameTimeMethod
Change from baseline mood15, 30, 45, 60, 90, and 120 minutes after meal consumption

Measured using visual analogue scale (mm). Each VAS is a 100 mm line where they will place a pencil mark to describe their feelings.

Change from baseline subjective appetite15, 30, 45, 60, 90, and 120 minutes after meal consumption

Measured using visual analogue scale (mm). Each VAS is a 100 mm line where they will place a pencil mark to describe their feelings.

Change from baseline glucose-dependent insulinotropic peptide (GIP)15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood GIP (pmol/L). GIP concentration in serum will be determined in duplicate via enzyme-linked immunosorbent assay kits (ELISA; Millipore, Billerica, Massachusetts).

Change from baseline glucagon-like peptide-1 (GLP-1)15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood GLP-1 (pmol/L). GLP-1 concentration in serum will be determined in duplicate via enzyme-linked immunosorbent assay kits (ELISA; Millipore, Billerica, Massachusetts).

Change from baseline cholecystokinin (CCK)15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood CCK (pmol/L). CCK concentration in serum will be determined in duplicate via enzyme-linked immunosorbent assay kits (ELISA; Millipore, Billerica, Massachusetts).

Change from baseline dipeptidyl peptidase 4 (DPP4)15, 30, 45, 60, 90, and 120 minutes after meal consumption

Blood DPP4 (ng/mL). DPP4 concentration in serum will be determined in duplicate via enzyme-linked immunosorbent assay kits (ELISA; Millipore, Billerica, Massachusetts).

Trial Locations

Locations (1)

School of Nutrition, Ryerson University

🇨🇦

Toronto, Ontario, Canada

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