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Specifying the Anti-inflammatory Effects of Ziltivekimab

Phase 3
Not yet recruiting
Conditions
Inflammation
Atherosclerosis
Interventions
Drug: Placebo
Registration Number
NCT06263244
Lead Sponsor
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)
Brief Summary

The goal of this randomized, double blind, placebo controlled trial is to study whether ziltivekimab therapy reduces arterial wall inflammation as assessed by imaging, and reduces the systemic inflammatory tone as assessed by circulating monocytes, inflammatory biomarkers and proteomics.

Detailed Description

Considering that ziltivekimab is currently undergoing a phase 3 CVOT trial, it is of great importance to elucidate its mechanistic effects. The objective of this study is to research whether ziltivekimab therapy for 20 weeks reduces arterial wall inflammation, as assessed by state-of-the-art imaging modalities, and reduces systemic inflammatory tone, as assessed by in depth phenotyping of circulating monocytes, inflammatory biomarkers and proteomics. The imaging modalities used in this study are 68Ga-DOTATATE PET/CT and CCTA. This study is designed as a single center, randomized, double-blinded, placebo-controlled intervention study in 40 atherosclerotic patients of 50 years and older with hsCRP levels of 2 mg/L and above.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
40
Inclusion Criteria
  • Aged 50 years and older.
  • Multi-vessel coronary artery disease (defined as CAD-RADS ≥2).
  • Serum hsCRP level ≥2 mg/L.
Exclusion Criteria
  • Coronary stents in situ.
  • Chronic or recent (<1 month) (serious) infections and/or clinical signs of acute (serious) infection.
  • History of severe auto-immune diseases, or other (severe) (recurrent or chronic) inflammatory disorders.
  • Use of preventive systemic antibiotics (antibiotics used to treat latent tuberculosis are exempted).
  • Stable lipid lowering treatment for less than 4 weeks, including statins, ezetimibe and PCSK9 inhibition.
  • Untreated latent tuberculosis, active hepatitis B (positive HBsAg and/or positive anti-HBc with detectable HBV DNA) or C, human immunodeficiency virus (HIV) not on stable antiretroviral regimen
  • Uncontrolled diabetes (HbA1c >90 mmol/mol).
  • Renal insufficiency, defined as eGFR <45 ml/min/1.73 m2.
  • Platelet count <120,000 and >450,000 /mm3.
  • Elevated liver enzymes (>3 ULN of liver transaminases), acute liver failure or known (severe) liver disease.
  • Premenopausal women not using birth-control.
  • History of gastrointestinal perforation, active diverticulitis (within 5 years) or active inflammatory bowel disease (within 12 months).
  • Uncontrolled hypertension (systolic >180 mmHg; diastolic >110 mmHg).
  • Diagnosis of (active) malignancy in last 5 years.
  • Standard contra-indications to 68Ga-DOTATATE PET, and CT based on physician's experience and current practices.
  • Inability or unwillingness to comply with the protocol requirements, or deemed by investigator to be unfit for the study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboPlacebo, subcutaneously, once per month for 5 months
ZiltivekimabZiltivekimab15 mg ziltivekimab subcutaneously once per month for 5 months
Primary Outcome Measures
NameTimeMethod
TBRmax coronary arteries5.5 months

mean percentage change in coronary arteries target to background ratio (TBRmax)

monocyte activation marker protein expression5.5 months

The impact of ziltivekimab on a mass cytometry monocyte phenotype panel; expression markers such as CD14 and CD16.

Secondary Outcome Measures
NameTimeMethod
delta TBRmax ascending aorta5.5 months

Difference in 68Ga-DOTATATE TBRmax of ascending aorta after treatment

delta PCAT5.5 months

Difference in PCAT (CCTA derived) after ziltivekimab treatment.

changes in hsCRP5.5 months

hsCRP (high-sensitivity C-reactive protein): mg/L

changes plasma cytokine and chemokine levels (pg/mL)5.5 months

TNF-α (Tumor Necrosis Factor alpha): pg/mL IL-8 (Interleukin-8): pg/mL MCP-1 (Monocyte Chemoattractant Protein-1): pg/mL

delta SUVmax bone marrow5.5 months

Difference in 68Ga-DOTATATE SUVmax of bone marrow after treatment.

changes monocyte phenotype5.5 months

The impact of ziltivekimab on monocyte phenotype in transendothelial migration (TEM) capacity and transcriptome profile.

Correlation delta TBRmax and CCTA derived plaque characteristics5.5 months

Correlation between changes in coronary 68Ga-DOTATATE uptake and anatomical plaque changes on CCTA.

changes plasma cytokine and chemokine levels (ng/mL)5.5 months

sICAM (soluble Intercellular Adhesion Molecule): ng/mL sVCAM (soluble Vascular Cell Adhesion Molecule): ng/mL

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