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The association between aging or chronic diseases and mitochondrial oxygenation and oxygen consumption as measured with the Protoporphyrin IX Triplet State Lifetime Technique

Recruiting
Conditions
Veroudering
aging
seniority
Registration Number
NL-OMON51166
Lead Sponsor
Anesthesiologie
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
Not specified
Target Recruitment
319
Inclusion Criteria

Healthy volunteers
In order to be eligible to participate in this study, a subject must meet all
of the following criteria:
- Aged between 18 years of age and 90 years of age
- Acceptable proficiency of the Dutch language
- Healthy volunteers without physical or mental illness

Comorbidity group
In order to be eligible to participate in this study, a subject must meet all
of the following criteria:
- Aged between 18 years of age and 90 years of age
- Acceptable proficiency of the Dutch language
- One of the underlying illnesses: neurodegenerative disease, diabetes mellitus
type II or obesity (BMI>30), no other relevant comorbidity

Exclusion Criteria

A potential subject who meets any of the following criteria will be excluded
from participation in this study:
- Porphyria
- Known intolerance to components of the ALA plaster
- Presence of mitochondrial disease
- Pregnancy/lactation
- Patients with skin lesions on the measurement location which impede
measurements
- Incapability to provide inform consent, due to a mental condition interfering
with the ability to understand the provided information

Study & Design

Study Type
Observational non invasive
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
<p>MitoPO2 (mmHg). Ten mitoPO2 (mmHg) baseline measurements will be performed on<br /><br>both the sternum and upper arm of all subjects. The mean and standard deviation<br /><br>of those measurements will compute the baseline mitoPO2 for every participant.</p><br>
Secondary Outcome Measures
NameTimeMethod
<p>MitoVO2 (mmHg/second). Applying pressure on the probe will cause a temporary<br /><br>halt of circulation to the mitochondria. As the oxygen consumption continues,<br /><br>this will result in a time-dependent decrease in mitoPO2 from which the mitoVO2<br /><br>can be calculated in mmHg/second. Three mitoVO2 measurements will be performed<br /><br>on both the sternum and the upper arm and the mean will be calculated for both.</p><br>
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