Effect of Continuous Versus High-intensity Interval Aerobic Training on the Muscular Vascular Adaptation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 25
- 试验地点
- 1
- 主要终点
- Percutaneous muscle biopsies of the vastus lateralis
研究概览
简要总结
The aim of this trial is to investigate the effect of aerobic training intensity on muscle vascular function: continuous versus interval.
详细描述
This trial will compare the hemodynamic effects of aerobic training on muscle microvascular function between participants randomly assigned to one of two groups: a continuous training group and an high-intensity interval training group.
All data concerning the primary and secondary outcomes will be obtained at the start and repeated at the end of 8 weeks aerobic training.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
盲法说明
Participants and the researcher will know the group allocation and therfore cannot be blinded.
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy female or male between 18 and 45 years
- •Be sedentary
- •Able to cycle
- •Able to provide written informed consent
排除标准
- •Have a pathology that may impact vascular function as :
- •cardiac pathology
- •pneumological pathology
- •metabolic pathology
研究组 & 干预措施
Moderate-Intensity Continuous Training (MICT): 34 min at 65% maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry), followed by 40 minutes of continuous cycling at 65% of their VO₂max. Sessions were conducted three times per week for a total duration of 8 weeks.
干预措施: Percutaneous muscle biopsies of the vastus lateralis (quadriceps) (Procedure)
Moderate-Intensity Continuous Training (MICT): 34 min at 65% maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry), followed by 40 minutes of continuous cycling at 65% of their VO₂max. Sessions were conducted three times per week for a total duration of 8 weeks.
干预措施: Measurement of the maximal oxygen uptake (VO2max) (Other)
Moderate-Intensity Continuous Training (MICT): 34 min at 65% maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry), followed by 40 minutes of continuous cycling at 65% of their VO₂max. Sessions were conducted three times per week for a total duration of 8 weeks.
干预措施: Non-Invasive Cardiac Output Measurement (Innocor) (Other)
High-Intensity Interval Training (HIIT): 2x2 min at 65% and 90% of maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry). The main training protocol consisted of alternating 2-minute cycling bouts: 2 minutes at 65% HRmax followed by 2 minutes at 90% HRmax, repeated continuously for a total of 28 minutes. Sessions were conducted three times per week over an 8-week period.
干预措施: Non-Invasive Cardiac Output Measurement (Innocor) (Other)
High-Intensity Interval Training (HIIT): 2x2 min at 65% and 90% of maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry). The main training protocol consisted of alternating 2-minute cycling bouts: 2 minutes at 65% HRmax followed by 2 minutes at 90% HRmax, repeated continuously for a total of 28 minutes. Sessions were conducted three times per week over an 8-week period.
干预措施: Percutaneous muscle biopsies of the vastus lateralis (quadriceps) (Procedure)
High-Intensity Interval Training (HIIT): 2x2 min at 65% and 90% of maximal heart rate
Participants performed a 3-minute warm-up at 50% of their maximal heart rate (HRmax), as determined during baseline cardiopulmonary exercise testing (ergospirometry). The main training protocol consisted of alternating 2-minute cycling bouts: 2 minutes at 65% HRmax followed by 2 minutes at 90% HRmax, repeated continuously for a total of 28 minutes. Sessions were conducted three times per week over an 8-week period.
干预措施: Measurement of the maximal oxygen uptake (VO2max) (Other)
结局指标
主要结局
Percutaneous muscle biopsies of the vastus lateralis
时间窗: At baseline and after completion of the 8-week intervention
Muscle tissue samples obtained from the vastus lateralis were processed using optical clearing and immunohistochemical staining techniques to visualize the microvascular network. Confocal microscopy was employed to acquire high-resolution three-dimensional images of arterioles, capillaries, and venules. Quantitative assessments included: 1. Internal diameter measurements, based on endothelial cell boundaries (intima layer) 2. External diameter measurements, based on smooth muscle cells and pericytes (media layer) 3. Capillary density, determined by counting the number of capillaries per sample volume
次要结局
- Cardiopulmonary Exercise Testing (CPET)(At baseline and after completion of the 8-week intervention)
- Maximal Cardiac Output Measurement Using Inert Gas Rebreathing (Innocor)(At baseline and after completion of the 8-week intervention)
研究者
Emilie Maufroy
Research Scientist, Reasearch Unit in Rehabilitation Sciences
Université Libre de Bruxelles