Antibacterial Effect of Ozonated Olive Oil, Propolis and Chlorhexidine Mouthwashes in Pediatric Patients

Not Applicable

Completed

• Conditions: Oral Hygiene; Oral Health

• Registration Number: NCT07197073
• Lead Sponsor: Alexandria University

Brief Summary

Streptococcus mutans and Lactobacillus acidophilus are the most common cariogenic microorganisms. Chlorhexidine has been the most widely used antibacterial mouthwash in dentistry. On the other hand, few studies have reported the antibacterial potential of Propolis and Ozonated olive oil as antibacterial agents that can be used as alternatives to Chlorhexidine. This study aims to evaluate and compare the antibacterial effect of Ozonated olive oil, Propolis, and Chlorhexidine on S. mutans and L. acidophilus count in pediatric patients.

Detailed Description

Not available

Study Timeline

• Study Start: 2025-02-25
• Primary Completion: 2025-05-10
• Study Completion: 2025-05-10
• Status Verified: 2025-09
• Study First Submitted: 2025-09-21
• Study First Submitted QC: 2025-09-21
• Last Update Submitted: 2025-09-21
• Study First Posted: 2025-09-29
• Last Update Posted: 2025-09-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 51

Inclusion Criteria

• High caries risk patients, according to AAPD guidelines, with visible caries or radiographic penetration into dentin, white spots on smooth surfaces, or recent restorations or missing teeth due to caries.
• Cooperative children according to Frankl's rating (score 3 & 4)

Exclusion Criteria

• Children with any medical illness or oral infection or those who received any type of antibiotic treatment or mouthwash for two weeks before or during the study were excluded

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Change in microbial count	Baseline and after 14 days of treatment	Total bacterial count before and after the intervention was detected. plaque samples were dispersed by vortexing for 30 seconds then 10 fold serially diluted using sterile saline.
Change in plaque accumulation	Baseline and after 14 days of treatment	The plaque accumulation was scored for each tooth on its four surfaces.The number of positively scored surfaces is divided by the total number of tooth surfaces evaluated and the result is multiplied by 100 to express the index as a percentage

Trial Locations

Locations (1)

Outpatient Clinic of Pediatric dentistry, Faculty of dentistry, Alexandria University, Egypt; 🇪🇬 Alexandria, Egypt