The effect of oral zinc supplementation on the T cells reconstitution in patients undergoing autologous hematopoietic stem cell transplantatio

Phase 2

Conditions: Patients with multiple myeloma who are candidates for stem cell transplantation..
Other and unspecified malignant neoplasms of lymphoid, hematopoietic and related tissue

Registration Number: IRCT20191211045701N1

Lead Sponsor: Tarbiat Modares University

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: All

Target Recruitment: 40

Inclusion Criteria

Age range 40 to 60 years
Patients with a history of multiple myeloma on complete response to treatment
Patients are candidates for autologous hematopoietic stem cell transplantation
Confirmation of the patient's heart, kidney, liver, lung, and digestive system health before hematopoietic stem cell transplantation by a specialist

Exclusion Criteria

History of sensitivity to zinc oral supplement
Patients who have taken oral supplements for three months before transplantation.
Serum levels above 200 mg/dl

Study & Design

Study Type: interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
umber of Recent Thymic Emigrant T-cells in peripheral blood mononuclear cells (PBMC). Timepoint: Before hematopoietic stem cell transplantation begging and 30, 90, 180 days after transplantation. Method of measurement: Using flow cytometry technique and specific RET T cells markers.;Determination of copy number of TREC (marker of thymus output) in peripheral blood mononuclear cells (PBMC). Timepoint: Before hematopoietic stem cell transplantation begging and 30, 90, 180 days after transplantation. Method of measurement: In DNA extracted from peripheral blood mononuclear cells using Real-Time-PCR.

Secondary Outcome Measures

Name	Time	Method
aive T cells number in the peripheral blood mononuclear cell population (PBMC). Timepoint: Before the hematopoietic stem cell transplantation process and 30, 90 and 180 days after transplantation. Method of measurement: Using flow cytometry techniques and T cells specific markers.;Memory T cells number in the peripheral blood mononuclear cell population (PBMC). Timepoint: Before the hematopoietic stem cell transplantation process and 30, 90 and 180 days after transplantation. Method of measurement: Using flow cytometry techniques and T cells specific markers.