Effect of Lactoferrin Administration on Rhabdomyolysis-induced Heart and Kidney Injury in Rats
试验速览
- 阶段
- 4 期
- 状态
- 已完成
- 发起方
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- AMPK levels
研究概览
简要总结
This study focuses on detecting the possible beneficial effect of Lactoferrin (Lf) administration on the kidney and the heart of glycerol-induced rhabdomyolysis (RM) in rats and its relation to ferroptosis and AMPK/Nrf2/ HO-1 signalling pathway. The main questions it aims to answer are:
- Is Lf administration 30 min. before or after RM induction in rats beneficial to kidneys and heart?
- When administered to glycerol-induced rats, does Lf act by upregulating the AMPK/Nrf2/ HO-1 signalling pathway and inhibiting ferroptosis?
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Triple (Participant, Care Provider, Outcomes Assessor)
入排标准
- 年龄范围
- 10 Weeks 至 12 Weeks（Child）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •• Twenty-four healthy male Wistar Albino rats weighing from 250 to 300 g
排除标准
- •No exclusion criteria as this study is used to detect effects of drugs on experimental rats.
研究组 & 干预措施
Control
Normal saline will be administered once to rats intramuscularly (10 mL/kg).
干预措施: iron (Diagnostic Test)
Control
Normal saline will be administered once to rats intramuscularly (10 mL/kg).
干预措施: MDA, TAC, and GSH (Diagnostic Test)
Control
Normal saline will be administered once to rats intramuscularly (10 mL/kg).
干预措施: Nrf2 (Diagnostic Test)
Control
Normal saline will be administered once to rats intramuscularly (10 mL/kg).
干预措施: AMPK (Diagnostic Test)
Control
Normal saline will be administered once to rats intramuscularly (10 mL/kg).
干预措施: HO-1 (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: iron (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: MDA, TAC, and GSH (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: Nrf2 (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: AMPK (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: HO-1 (Diagnostic Test)
RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage.
干预措施: glycerin (Drug)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: Lactoferrin (Drug)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: iron (Diagnostic Test)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: MDA, TAC, and GSH (Diagnostic Test)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: Nrf2 (Diagnostic Test)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: AMPK (Diagnostic Test)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: HO-1 (Diagnostic Test)
Lf-RM
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction
干预措施: glycerin (Drug)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: Lactoferrin (Drug)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: iron (Diagnostic Test)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: MDA, TAC, and GSH (Diagnostic Test)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: Nrf2 (Diagnostic Test)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: AMPK (Diagnostic Test)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: HO-1 (Diagnostic Test)
RM-Lf
A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction.
干预措施: glycerin (Drug)
结局指标
主要结局
AMPK levels
时间窗: 2 months
AMPK levels in kidney and heart tissue homogenate
HO-1 levels
时间窗: 2 months
HO-1 levels in kidney and heart tissue homogenate
Nrf2 expression
时间窗: 2 months
Nrf2 expression in heart and kidney tissues
次要结局
- GSH levels(2 months)
- TAC levels(2 months)
- MDA levels(2 months)
- iron levels(2 months)
研究者
Asmaa Abdel-mageed Muhammed
Lecturer
Aswan University Hospital