The incorporation of dietary protein derived amino acids in duodenal epithelium in young and older males.

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 16

Inclusion Criteria

- Male sex
- Aged 18-35 years or 67+ years
- Body mass index (BMI) between 18.5 and 30 kg/m2

Exclusion Criteria

- History of cardiovascular, respiratory, gastrointestinal, urogenital,
neurological, psychiatric, dermatologic, musculoskeletal, metabolic, endocrine,
haematological, immunologic disorders, allergy, major surgery and/or laboratory
assessments which might limit participation in or completion of the study
protocol, interfere with the execution of the experiment, or potential
influence the study outcomes (to be decided by the principal investigator and
responsible physician)
- Major abdominal surgery interfering with gastrointestinal function (upon
judgement of the principal investigator and responsible physician)
- Use of medication which limit participation in or completion of the study
protocol, interferes with the execution of the experiment, or potential
influences the study outcomes (to be decided by the principal investigator and
responsible physician)
- Use of supplementation (i.e. vitamin, pre- and probiotic supplementation)
within 14 days prior to testing
- Administration of investigational drugs or participation in any scientific
intervention study in the 14 days prior to the study, which may interfere with
this study (upon judgement of the principal investigator and responsible
physician)
- Specific diet (e.g. vegetarian, vegan, gluten free, no diary) within the
study period
- Planning to lose weight during the study period
- Lactose intolerance
- Excessive alcohol consumption (defined as > 14 alcoholic consumptions per
week)
- Smoking
- Drug use
- Donated blood two months prior to the test day
- Recent (<1 year) participation in amino acid tracer
(L-[ring-2H5]-phenylalanine, L-[ring-2H3]-leucine, L-[ring-2H4]-lysine,
L-[ring-2H2]-tyrosine) or intrinsically labelled protein
([1-13C]-phenylalanine, [1-13C]-leucine, [1-13C]-lysine) studies
- No given permission to register participation in electronic patient file at
MUMC+ and to add records of gastroduodenoscopy

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. To assess the postprandial (0-5h) incorporation of dietary protein derived<br /><br>amino acids in duodenal epithelium in healthy young and older males.<br /><br>2. To investigate whether age affects the postprandial (0-5h) incorporation of<br /><br>dietary protein derived amino acids in duodenal epithelium. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1. To assess the postprandial (0-5h) plasma availability of dietary protein<br /><br>derived amino acids in healthy young and older males.<br /><br>2. To assess postprandial (0-5h) fractional duodenal mucosal and muscle protein<br /><br>synthetic rates in healthy young and older males.</p><br>