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Comparision of Pharmacokinetics(PK) and Pharmacodynamics(PD) of Biocon Insulin R and Humulin® R

Phase 1
Completed
Conditions
Healthy Volunteer
Interventions
Biological: Humulin®R
Biological: Biocon Insulin R
Registration Number
NCT04022317
Lead Sponsor
Biocon Limited
Brief Summary

Single-centre, randomised, double-blind, single dose, two-treatment, two-period, two sequence, crossover,12-hour euglycaemic glucose clamp trial in healthy subjects

Detailed Description

The present study is designed to demonstrate pharmacokinetic and pharmacodynamic equivalence of Biocon Insulin R with Humulin® R in healthy subjects.

The treatment consists of one single dose of the test or reference product, administered during each of the two study periods, separated by 5-7 days between each dosing.

The planned trial duration for each subject is about 12 to 36 days. Eligible subjects will undergo two 12-hour euglycaemic clamp examinations, one after administration of the test product and one after administration of the reference product in random order.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
42
Inclusion Criteria
  • Healthy male or post-menopausal female subject. Post-menopausal state is defined as no menses for 12 months without an alternative medical cause and confirmed by a follicle stimulating hormone (FSH) level in the post-menopausal range (>= 25.8 IU/L).
  • Age between 18 and 55 years, both inclusive.
  • Body Mass Index (BMI) between 18.5 and 29.0 kg/m^2, both inclusive.
  • Fasting plasma glucose concentration <= 100 mg/dL.
  • Considered generally healthy upon completion of medical history and screening safety assessments, as judged by the Investigator
Exclusion Criteria
  • Known or suspected hypersensitivity to Investigational Medicinal products (IMP(s)) or related products.
  • Receipt of any medicinal product in clinical development within 30 days or five times its half-life (whichever is longer) before randomisation in this trial.
  • Any history or presence of clinically relevant comorbidity, as judged by the investigator.
  • Systolic blood pressure < 95 mmHg or >140 mmHg and/or diastolic blood pressure < 50 mm Hg or > 90 mmHg after resting for at least 5 minutes in supine position (excluding white-coat hypertension; therefore, a repeat test showing results within range will be acceptable).
  • Pulse rate at rest outside the range of 50-90 beats per minute.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Humulin® R (regular insulin human)Humulin®R0.3 IU/kg Dose per administration, subcutaneous Route of administration
Biocon Insulin RBiocon Insulin R0.3 IU/kg Dose per administration, subcutaneous Route of administration
Primary Outcome Measures
NameTimeMethod
PD endpoints: Area under the glucose infusion rate curve(AUCGIR).0-12h0-12 hours

Area under the glucose infusion rate curve

PD endpoints:maximum glucose infusion rate(GIRmax)0-12 hours

maximum glucose infusion rate

PK endpoints:Area under the insulin concentration curve(AUCins).0-12h0-12 hours

Area under the insulin concentration curve from 0 to 12 hours.

PK endpoints: Maximum observed insulin concentration(Cins.max)0-12 hours

Maximum observed insulin concentration

Secondary Outcome Measures
NameTimeMethod
PK endpoint- time(t)50%-ins(late)0-12 hours

time to half-maximum after Cins.max.

PD endpoint: area under the glucose infusion rate curve(AUCGIR).0-2h0 to 2 hours

area under the glucose infusion rate curve

PK endpoint- Area under the insulin concentration curve(AUCins).0-2h0 to 2 hours

Area under the insulin concentration curve

PK endpoint- Area under the insulin concentration curve(AUCins).0-6h0 to 6 hours

area under the insulin concentration curve

PK endpoint- Area under the insulin concentration curve(AUCins).0-infinity0 hours to 24 hours

area under the insulin concentration-time curve

PD endpoint: area under the glucose infusion rate curve(AUCGIR).6-12h6 to 12 hours

area under the glucose infusion rate curve

PK endpoint- Area under the insulin concentration curve(AUCins).6-12h6 to 12 hours

area under the insulin concentration curve

PK endpoint- time(t)50%-ins(early)0-12 hours

time to half-maximum before Cins.max.

PK endpoint- terminal elimination half-life (t½)0-12 hours

terminal elimination half-life calculated as t½=ln2/λz.

PD endpoint: time to maximum glucose infusion rate (tGIR.max)0-12 hours

time to maximum glucose infusion rate

PD endpoint: time to half-maximum glucose infusion rate before GIRmax (tGIR,50%-early0-12 hours

time to half-maximum glucose infusion rate before GIRmax

PK endpoint- time to maximum concentration( tmax)0-12 hours

time to maximum observed insulin concentration.

PK endpoint- terminal elimination rate constant (λz)0-12 hours

terminal elimination rate constant of insulin.

PD endpoint: area under the glucose infusion rate curve(AUCGIR).0-6h0 to 6 hours

area under the glucose infusion rate curve

PD endpoint:time to half-maximum glucose infusion rate after GIRmax (tGIR.50%-late)0-12 hours

time to half-maximum glucose infusion rate after Maximum glucose infusion rate(GIRmax)

PD endpoint: Onset of action0-12 hours

ime from trial product administration until blood glucose concentration has decreased at least 5 mg/dL from baseline, where baseline is defined as the mean of blood glucose levels from -6, -4, and -2 minutes before trial product administration as measured by ClampArt®((name of Clamp Devise)

Trial Locations

Locations (1)

Profil Mainz GmbH

🇩🇪

Mainz, Germany

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