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A Phase I Study of an HIV Vaccine in Healthy, HIV Uninfected Adults

Phase 1
Completed
Conditions
Healthy
Interventions
Biological: MVA Mosaic
Biological: Placebo
Registration Number
NCT02218125
Lead Sponsor
Crucell Holland BV
Brief Summary

The purpose of this study is to assess the safety and tolerability of Modified Vaccinia Ankara (MVA) Mosaic vaccine in healthy adult participants.

Detailed Description

This is a Phase I, placebo-controlled (the use of an inactive substance identical in appearance to the active vaccine), double-blind (neither the participant or study personnel will know the identity of the treatment administered) study where participants will be randomized (treatment type assigned by chance) to receive a Modified Vaccinia Ankara (MVA) Mosaic vaccine (at 1x10E8 pfu) or placebo. This design is intended to reduce the likelihood of observer and selection bias, provide control for confounding variables, and aid an unbiased analysis of the study results. The study will include 4 groups of participants, 2 groups having previously been vaccinated with Ad26.ENVA.01 (A recombinant adenovirus \[rAd\] vaccine for HIV-1) and 2 groups not previously vaccinated with Ad26.ENVA.01. Participants will be randomized in a 4:1 ratio to receive either a MVA Mosaic vaccine or placebo. The trial comprises a 4-week screening period, a 12-week vaccination period during which participants will be vaccinated at baseline (Day 1) and Week 12 (Day 84), and a 40-week follow-up period to the final visit at Week 52.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
25
Inclusion Criteria
  • Healthy adults (determined by medical history, physical examination, and clinical judgment)
  • HIV uninfected
  • Female participants of child bearing potential must have a negative serum β-human chorionic gonadotrophin pregnancy test at the screening visit and immediately prior to each vaccine/placebo administration, practice adequate birth control measures from 28 days prior to the first vaccine/placebo administration through to at least 3 months after the final vaccine/placebo administration
  • Male participants who are sexually active with a woman of childbearing potential and has not had a vasectomy must agree to use a double barrier method of birth control, e.g. either condom with spermicidal foam/gel/film/cream/suppository or partner with occlusive cap (diaphragm or cervical/vault caps) with spermicidal foam/gel/film/cream/suppository
Exclusion Criteria
  • Confirmed HIV-1/-2 infection
  • Chronic active hepatitis B or hepatitis C or active syphilis infection. Active syphilis documented by exam or serology unless positive serology is due to past treated infection
  • Within the 12 months prior to enrollment: a history of newly acquired syphilis, gonorrhea, non-gonococcal urethritis, herpes simplex virus type 2 (HSV2), Chlamydia, pelvic inflammatory disease (PID), trichomonas, mucopurulent cervicitis, epididymitis, proctitis, lymphogranulomavenereum, chancroid, or hepatitis B
  • A woman who is breastfeeding
  • Any clinically significant acute or chronic medical condition that, in the opinion of the investigator, would preclude participation
  • Major surgery within the 4 weeks prior to study entry or planned major surgery through the course of the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Group 1- MVA MosaicMVA MosaicHealthy volunteers not previously vaccinated with Ad26.ENVA.01 will be administered Modified Vaccinia Ankara (MVA) Mosaic at Week 0 and at Week 12.
Group 3 - MVA MosaicMVA MosaicHealthy volunteers previously vaccinated with Ad26.ENVA.01 will be administered MVA Mosaic at Week 0 and at Week 12.
Group 2 - PlaceboPlaceboHealthy volunteers not previously vaccinated with Ad26.ENVA.01 will be administered placebo at Week 0 and at Week 12.
Group 4- PlaceboPlaceboParticipants previously vaccinated with Ad26.ENVA.01 will be administered placebo at Week 0 and at Week 12.
Primary Outcome Measures
NameTimeMethod
The Number of Participants who Experience Adverse Events Within 28 days After VaccinationFor 28 days following vaccination on Day 1 and Day 84

In addition to the number of participants who experience adverse events within the time frame of 28 days after each vaccination, all adverse events that occur from the signing of the informed consent through to the last study visit (Visit 10 \[Day 365\]) will be reported.

The Number of Participants who Experience Reactogenicity Symptoms Following Vaccination1 week following vaccination on Day 1 and Day 84

Reactogenicity symptoms monitored following vaccination will include erythema (redness), induration (hardening), and local pain/tenderness, itching, swelling, or warmth at the site of injection, temperature (fever \>37.7 degree Celsius); fatigue (extreme tiredness), headache, myalgia (muscle pain), arthralgia (joint pain), chills, nausea, vomiting, rashes, and itching (general and local)

Secondary Outcome Measures
NameTimeMethod
The Number of Participants With Humoral Immune Responses4 weeks after the second vaccination

Humoral immune responses will be evaluated by the detection of env-specific binding antibody.

Durability of Immune Response6, 9, and 12 months after the first vaccination

Determined by humoral immune response assays.

The Number of Participants With T-cell Responses Following Vaccination4 weeks after the second vaccination

T-cell responses to be determined by epitope mapping.

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