Treatment of Chronic Periapical Periodontitis of Young Permanent Teeth by Means of Three Adjuvant Treatment Approaches: A Prospective Comparative Microbiological and Clinical Study
Overview
- Phase
- Not Applicable
- Intervention
- Laser-device group
- Conditions
- Treatment
- Sponsor
- University of Belgrade
- Enrollment
- 40
- Locations
- 1
- Primary Endpoint
- Number of teeth without bacteria load after laser-devices and essential oil in endodontic treatment
- Status
- Completed
- Last Updated
- 4 years ago
Overview
Brief Summary
To determine microbiology assessment of infected root canal in young permanent teeth, as well as antimicrobial efficiency of different adjuvant treatment modalities including photodynamic therapy (PDT), high-power diode laser, and essential oils following mechanic-chemical treatment in the treatment of periapical periodontitis in these teeth.
Detailed Description
To determine antimicrobial efficiency of adjuvant photodynamic therapy (PDT) and high-power diode laser, and essential oils in the treatment of chronic periapical periodontitis in young permanent teeth. Material and Methods: Young permanent teeth with chronic periapical periodontitis were selected and randomly divided into tests and control groups. Adjuvant treatment approaches were randomly performed following standard mechanical-chemical endodontic treatment: 1. Experimental laser-device 1, PDT group: adjuvant PDT (n=10) 2. Experimental laser-device 2, Diode laser (940 nm, 1W) (n=10) 3. Experimental essential oil (n=10) 4. Experimental sodium hypochlorite (NaoCL) group (n= 10) solely mechanical instrumentation with 1.5 % of NaOCl irrigation was performed (positive control). Afterwards, each root canal was filled with calcium hydroxide, and definitive obturation was done seven days respectively. Microbiological analyses of root canals were assessed after accessing the canal (sample 1), following mechanical-chemical treatment (sample 2), and adjuvant treatment approaches (sample 3). The microbiological identification and quantification were provided by MALDI -TOF spectrometry and plate count assay. To evaluate the efficiency of treatment modalities, radiography records were additionally used.
Investigators
Dragana Rakasevic
Principal Investigator and Research Associate
University of Belgrade
Eligibility Criteria
Inclusion Criteria
- •Had signed informed consent by the parents or legal guardian ;
- •Systemically healthy children;
- •Had not been submitted to antibiotic therapy in the previous 3 months;
- •Had a least one young permanent anterior tooth (incisor or canine) or the first premolar with pulp necrosis and presence of a chronic periapical lesion (CPP);
- •Had no endodontic treatment of the selected tooth;
- •Had no signs of an acute condition;
Exclusion Criteria
- •Presence of systemic diseases such as diabetes mellitus, asthma, chronic infections, immunosuppressive diseases or other that could compromise the patient immune system
- •Use anti-inflammatory or immunosuppressive therapy in the last 6 months;
- •Patients with antibiotic prophylactic requirements before dental treatment;
Arms & Interventions
Laser-devices group
Mechanico-chemical root canal preparation and root canal disinfection were be performed by means of mechanic-chemical methods including hand instruments followed by 1.5% sodium hypochlorite. Afterwards, depending on allocations, in the laser devices group, adjuvant treatment approaches were be performed by means of laser devices either photodynamic therapy (HELBO, Photodynamic Systems GmbH), or high power diode laser (940nm, Biolase).
Intervention: Laser-device group
Laser-devices group
Mechanico-chemical root canal preparation and root canal disinfection were be performed by means of mechanic-chemical methods including hand instruments followed by 1.5% sodium hypochlorite. Afterwards, depending on allocations, in the laser devices group, adjuvant treatment approaches were be performed by means of laser devices either photodynamic therapy (HELBO, Photodynamic Systems GmbH), or high power diode laser (940nm, Biolase).
Intervention: Sodium hypochlorite group
Sodium hypochlorite
Mechanico-chemical root canal preparation and root canal disinfection were be performed by means of mechanic-chemical methods including hand instruments followed by 1.5% sodium hypochlorite (pH 12), at room temperature (21 degrees Celsius).
Intervention: Sodium hypochlorite group
Essential oil
Mechanico-chemical root canal preparation and root canal disinfection were be performed by means of mechanic-chemical methods including hand instruments followed by 1.5% sodium hypochlorite (pH 12), at room temperature (21 degrees Celsius). Depending on allocations, in the essential oil group, the canals were be treated with adjuvant essential oil of Cymbopogon martinii and Thymus vulgaris (Herba oils, Herba doo, Belgrade, Serbia)
Intervention: Essential Oil
Essential oil
Mechanico-chemical root canal preparation and root canal disinfection were be performed by means of mechanic-chemical methods including hand instruments followed by 1.5% sodium hypochlorite (pH 12), at room temperature (21 degrees Celsius). Depending on allocations, in the essential oil group, the canals were be treated with adjuvant essential oil of Cymbopogon martinii and Thymus vulgaris (Herba oils, Herba doo, Belgrade, Serbia)
Intervention: Sodium hypochlorite group
Outcomes
Primary Outcomes
Number of teeth without bacteria load after laser-devices and essential oil in endodontic treatment
Time Frame: 6 months
The number of teeth without isolated bacteria load were assessed. Microbiological samples from the root canals were collected immediately after accessing the canal, following endodontic treatment, and after the adjuvant treatments in allocated groups (Photodynamic therapy or Diode laser or Essential oil). The microbiological identification and quantification were provided by MALDI -TOF spectrometry and plate count assay.
Number of teeth without bacteria load after sodium hypochlorite (NaOCl) in endodontic treatment
Time Frame: 6 months
The number of teeth without isolated bacteria load were assessed after sodium hypochlorite irrigation in each group (Laser-device, Essential oil and Sodium hypochlorite). Microbiological samples from the root canals were collected immediately after accessing the canal, following the endodontic treatment. The microbiological identification and quantification were provided by MALDI -TOF spectrometry and plate count assay.
Secondary Outcomes
- Radiography record of periapical lesion healing(Change baseline radiography record at 6 months)