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Effect of a Probiotic Mixture on the Gut Microbiome and Fatigue in Patients With Quiescent Inflammatory Bowel Disease

Not Applicable
Completed
Conditions
Crohn Disease
Ulcerative Colitis
Inflammatory Bowel Diseases
Interventions
Dietary Supplement: Placebo
Dietary Supplement: Probiotic Mixture
Registration Number
NCT03266484
Lead Sponsor
Massachusetts General Hospital
Brief Summary

The study is proposed as a single-site randomized double-blind placebo-controlled trial requiring 4 study visits, where two of the visits are combined with their appointment for routine clinical care. The study population will consist of patients with quiescent CD and UC and IBD-unspecified recruited from the Massachusetts General Hospital Crohn's and Colitis center. All eligible subjects will have a confirmed diagnosis of CD, UC, or IBD-unspecified according to accepted clinical, endoscopic, radiologic, and histologic criteria. Eligible patients will be contacted at the time of their routinely scheduled office visit and consented for the study. Self-report and review of medical records will be used to obtain detailed information regarding their disease on an intake questionnaire completed by a research study coordinator.

The study is proposed as a 12-week double-blind randomized controlled trial of the probiotic supplement compared to placebo. We propose to examine the effect of a specific probiotic supplement on the changes in the gut microbiome, serum metabolomic profile, and fatigue symptoms in patients with quiescent IBD.

Within 2 weeks of screening, eligible patients will be invited to visit MGH for a baseline visit. The patient will receive either the probiotic supplement or placebo for 4 weeks.

The first follow up visit will be at week 4 to check for adverse events on study treatment, to check accountability of probiotics/placebo, and to complete the set of questionnaires. Also, subjects will receive probiotic/placebo samples for the remaining 8 weeks of treatment.

At week 8, subjects will receive a phone call from a study research coordinator to check in with probiotics/placebo intake for treatment compliance and accountability records, and to complete the set of questionnaires ascertaining subjective symptoms.

Last study visit will be at week 12 which is often combined with a regular office appointment. Subject will provide serum blood and stool sample, as well as complete the set of questionnaires.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
100
Inclusion Criteria
  • Age 18-75 years
  • Confirmed CD or UC or IBD-unspecified diagnosed according to standard criteria
  • Quiescent disease defined as Harvey Bradshaw index < 4 at baseline (week 0) or SCCAI < 2
  • Persistent ongoing fatigue symptoms
  • Endoscopic or radiologic remission within 12 months of screening
  • Washout of non-study probiotic supplements for at least 4 weeks prior to screening.
Exclusion Criteria
  • Patients with clinical or endoscopically active inflammatory bowel disease
  • Significant non-IBD comorbidity contributing to the fatigue (such as active cancer).
  • Untreated severe depression or anxiety
  • Known sleep disorders without adequate treatment,
  • Presence of J-pouch or a stoma
  • Ongoing use of other non-study probiotics
  • Women who are pregnant or lactating

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboParticipants who are meeting the inclusion criteria will be randomized to either the probiotic mixture or an identical placebo The identical placebo sachets will be taken twice a day for 12 weeks.
Probiotic MixtureProbiotic MixtureParticipants who are meeting the inclusion criteria will be randomized to either the probiotic mixture or an identical placebo. The probiotics sachets will be taken twice a day for 12 weeks.
Primary Outcome Measures
NameTimeMethod
Change in gut microbiomeWeek 12

Change in fecal microbiome pattern assessed using 16sRNA sequencing.

Change in serum inflammatory cytokines levelsWeek 12

Change in inflammatory cytokines will be performed using the Biosciences Cytometric Bead Array kits.

Change in metabolomic profilesWeek 12

Change in metabolic profiling will be performed on serum samples using established targeted quantitative and semi-quantitative gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) methods

Change in fatigue symptomsWeek 4 and Week 12

Reduction of fatigue symptoms which will be assessed using FACIT-F questionnaire. FACIT-F score of \>43 at week 4 or week 12

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Crohn's and Colitis Center, MGH

🇺🇸

Boston, Massachusetts, United States

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