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Study to Evaluate Hepatic Artery Injection of Autologous Human Bone Marrow-Derived MSCs in Patients With Alcoholic LC

Phase 1
Conditions
Alcoholic Liver Cirrhosis
Interventions
Biological: Cellgram™ (Bone marrow-derived MSCs)
Registration Number
NCT03838250
Lead Sponsor
Pharmicell Co., Ltd.
Brief Summary

This study is to evaluate the safety and efficacy of a single dose of Cellgram™ delivered via hepatic artery in patients with decompensated alcoholic liver cirrhosis.

Detailed Description

After providing written informed consent, subjects will return to the study center for further evaluation and to have their Bone marrow harvested by an experienced hematologist or interventional radiologist.

Within approximately 1 month (30 ± 7 days) after Bone marrow aspiration, study participants will be admitted to the study center on Day 1.

At the study center, the participant will undergo hepatic artery catheterization by an interventional radiologist who will inject a single dose of Cellgram™. Participants will remain as in-patients and be observed for 24 hours post-infusion. Following discharge, participants will periodically return to the study center for study assessment visits over a period of 1 year.

When a suitable candidate is identified by the Investigator, the Investigator or designated healthcare professional will ask the patient about his/her willingness to be included in the clinical study. Following this, patients will be allowed sufficient time, in their own opinion, to consider study entry, and will be offered the opportunity to ask any further questions prior to signing the informed consent form.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
10
Inclusion Criteria
  1. Alcoholic liver cirrhosis as diagnosed by clinical, biochemical, radiological, or histological evidence.
  2. Male or female, 18 to 70 years of age, inclusive.
  3. Child-Pugh class B (7 to 9 points)
  4. Capable, in the Investigators opinion, of undergoing hepatic artery catheterization.
  5. No consumption of alcohol or other potentially hepatotoxic substances considered clinically relevant in the opinion of the Investigator, within 6 months prior to screening and throughout the study.
  6. Provision of informed consent by the patient (or their legal representative) to participate in the clinical study.
  7. Able, in the Investigator's opinion, to comply with the requirements of the protocol (including the follow-up period).
  8. Females of childbearing potential must test negative on standard urine pregnancy test and must be willing to practice appropriate contraceptive methods for the duration of the study. Highly effective methods of birth control include hormonal birth control, intrauterine devices (IUDs), or any double-barrier method (sponges, female condoms) used by the woman in addition to contraception used by their male partner such as vasectomy or condom supplemented with spermicide.
Exclusion Criteria
  1. Current diagnosis of malignant hematologic disease (e.g., acute myeloid leukemia, acute lymphoblastic leukemia, non-Hodgkin's lymphoma, Hodgkin's lymphoma, multiple myeloma).

  2. Etiology other than alcohol for underlying liver cirrhosis.

  3. Baseline creatinine >1.7 mg/dL and/or estimated glomerular filtration rate (eGFR) <30 mL/min/1.73 m2

  4. Clinical history of a solid cancer within 5 years prior to screening or current diagnosis of a solid cancer (including hepatocellular carcinoma assessed by ultrasonography and elevated AFP level) and currently receiving cancer treatment.

    Continuous use of a clinically relevant amount of steroids or antibiotics within 1 month prior to screening. Clinical relevance will be determined by the Investigator.

  5. Model for End-Stage Liver Disease score >20.

  6. International normalized ratio >3.0 and/or platelet counts <30,000/mm3

  7. Major operation within 3 months prior to screening.

  8. Presence of extrahepatic biliary stricture.

  9. Participant has undergone transjugular intrahepatic portosystemic shunt.

  10. Active hepatic artery or portal vein thrombosis.

  11. Presence of advanced hepatic encephalopathy Stages 3-4 (West Haven criteria) at the time of screening.

  12. Active variceal bleeding during the last 6 months before screening.

  13. Severe cardiac, renal, or respiratory failure.

  14. Positive serological test results for human immunodeficiency virus (HIV), HCV, hepatitis B surface antigen (HBsAg) and/or syphilis.

  15. Patient is pregnant or breast feeding, or planning to become pregnant while enrolled in the study, up to the EOS visit.

  16. Positive urine pregnancy test at Screening.

  17. Drug abuse within the past 2 years (as confirmed by patient disclosure or a urine drug screen conducted at Screening).

  18. Participation in an interventional clinical study within 30 days prior to screening.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Cellgram™ (Bone marrow-derived MSCs)Cellgram™ (Bone marrow-derived MSCs)Infusion Cellgram™(Bone marrow-derived MSCs). Single dose administration of approximately 5 x 10\^7 cells/10 mL (range: 4.5 x 10\^7 to 5.5 x 10\^7 cells/10 mL) via the hepatic artery.
Primary Outcome Measures
NameTimeMethod
Incidence of Serious Adverse Events12 months

An SAE suggests a significant hazard, contraindication, side-effect, or precaution. With respect to human clinical experience, this includes any event that:

* Results in death.

* Is life-threatening.\*

* Requires in-patient hospitalization or prolongation of existing hospitalization.

* Results in persistent or significant disability/incapacity.

* Is a congenital anomaly/birth defect.

* Other medically important condition

* Life-threatening in the definition of a SAE or adverse reaction refers to an event in which the patient was at risk of death at the time of event; it does not refer to an event, which hypothetically might have caused death if it were more severe.

Secondary Outcome Measures
NameTimeMethod
Incidence of Adverse Events12 months

Incidence of Adverse Events as assessed by vital signs, physical examination, safety laboratory tests, and patient reporting

Liver stiffness measurement12 months

with transient elastography (i.e., FibroScan®)

Number of patients with Hepatocellular carcinoma (primary liver cancer) development12 months

assessed via ultrasonography and alpha fetoprotein \[AFP\] analysis

Overall survival12 months

defined as the time from infusion until death from any cause during the study period.

Quality of life as assessed by 36-Item Short Form Survey (SF-36) Questionnaire12 months

The Short Form-36 Quality Of Life questionnaire will be recorded for each patient. The 8 subscales of the SF-36 (Physical Functioning, Role Physical, Bodily Pain, General Health, Vitality, Social Functioning, Role Emotional, Mental Health) and 2 summary component measures (Physical Component Summary, Mental Component Summary) will be calculated and summarized.

Model for End-Stage Liver Disease (MELD) Score12 months

MELD uses the patient's values for serum bilirubin, serum creatinine, and the INR for PT to predict survival. It is calculated according to the following formula defined by Kamath et al (2001):

MELD = 3.78×ln\[serum bilirubin (mg/dL)\] + 11.2×ln\[INR\] + 9.57×ln\[serum creatinine (mg/dL)\] + 6.43

How well the Liver is functioning12 months

by tests including: serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin, albumin, gamma glutamyl transpeptidase (GGT) and creatinine.

Units of Measure: AST will be report in U/L. ALT will be report in U/L. GGT will be report in U/L. Bilirubin will be report in mg/dL. creatinine will be report in mg/dL. Albumin will be report in g/dL

Chronic liver disease as assessed by the Child-Pugh score12 months

The Child-Pugh Score will be used to determine the prognosis, required strength of treatment and the necessity of liver transplantation. The score employs 5 clinical measures of liver disease (total bilirubin, serum albumin, INR, ascites and hepatic encephalopathy). Each parameter is scored 1 to 3, with 3 indicating the most severe derangement.

Trial Locations

Locations (1)

University of Utah

🇺🇸

Salt Lake City, Utah, United States

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