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Pediatric Burn Treatment Using Tilapia Skin as a Xenograft for Superficial-Partial Thickness Wounds

Phase 2
Completed
Conditions
Burns
Interventions
Registration Number
NCT04391582
Lead Sponsor
Nucleo De Pesquisa E Desenvolvimento De Medicamentos Da Universidade Federal Do Ceara
Brief Summary

This study aims to evaluate the efficacy of Nile tilapia skin as a xenograft for the treatment of partial-thickness burn wounds in children.

Detailed Description

This is an open-label, monocentric, randomized phase II pilot study conducted in Fortaleza, Brazil. The study population consisted of 30 children between the ages of 2 and 12 years with superficial "partial-thickness" burns admitted less than 72 hours from the thermal injury. In the test group, the tilapia skin was applied. In the control group, a thin layer of silver sulfadiazine cream 1% was applied.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Children between the ages of 2 and 12 years admitted to our institution with superficial partial thickness burns less than 72 hours from the thermal injury
Exclusion Criteria
  • Patients were excluded for a burn greater than 15% total body surface area (TBSA);
  • Presence of a previous treatment for the current burn;
  • Presence of a chemical or electrical burn;
  • Presence of other significant diseases that could impact the volunteer's participation in the study;
  • Presence of hypersensitivity to materials used in the study or to related compounds.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Control - Silver sulfadiazinesilver sulfadiazine cream 1%After cleaning the lesion with tap water and 2% chlorhexidine gluconate, a thin layer of silver sulfadiazine cream 1% was applied and covered with gauze and band
Test - Tilapia SkinTilapia skinAfter cleaning the lesion with tap water and 2% chlorhexidine gluconate, the tilapia skin was applied and covered with gauze and bandage.
Primary Outcome Measures
NameTimeMethod
Assessment of pain via the Faces Pain Scale-Revised (FPS-R).Day 11

The pain evaluation via the FPS-R was performed by the patient himself or herself if aged 5 years or more, or by their caregiver in patients under 5 years of age. The scale consists of six faces, presented horizontally, that depict different degrees of pain, from "no pain" to "most pain possible". A numerical value from 0 to 10 is assigned to each face.

Assessment of pain via FLACCr scale.Day 11

The pain evaluation via FLACCr scale was performed by the physician responsible and answered five evaluation categories based on behavioral parameters with combined scores ranging from zero to ten (classified the scores as follows: zero to three (mild pain); four to six (moderate pain) and seven to ten (severe pain).

Complete burn wound healingDay 11

The number of days to complete burn wound healing (≥95% reepithelialization), calculated via clinical judgment from the consultant.

Assessment of pain via CONFORT-B scale.Day 11

The pain evaluation via CONFORT-B Scale was performed by the physician responsible and assessed the pain based in a behavioral that consisted in six factors: alertness, calmness/agitation, respiratory response (or crying), physical movement, muscle tone, and facial tension.Each factor was scored with values ranging between 1 and 5, generating scores between 6 and 30 points.

Secondary Outcome Measures
NameTimeMethod
Evaluation of burn severity via the Clinical Global Impression Scale- Severity (CGI-S).Day 1, day 3, day 5, day 7, day 9 and day 11

The CGI-I was evaluated by the physician responsible and answers the following question: "Compared to the patient's condition at admission to the project, this patient's condition is: 1=very much improved since the initiation of treatment; 2=much improved; 3=minimally improved; 4=no change from baseline (the initiation of treatment); 5=minimally worse; 6= much worse; 7=very much worse since the initiation of treatment."

Number of dressings performed.Day 11

In the test group, a dressing change was defined as the act of replacing the tilapia skin that did not adhere properly and/or replacing gauze and bandage that is full of exudate, after an evaluation of the dressing (performed every 48 hours). In the control group, a dressing change was defined as the daily act of cleaning the wound and reapplying the silver sulfadiazine cream 1%, which is then covered with new gauze and bandage.

Total amount of anesthetics and analgesics required throughout the treatmentDay 11

To audit anesthetics and analgesics intake, nurses were trained to register on the patient's clinical record all analgesic and anesthetic medications used

Evaluation of burn improvement via the Clinical Global Impression Scale-Improvement (CGI-I).Day 3, day 5, day 7, day 9 and day 11

The CGI-I was evaluated by the physician responsible and answers the following question: "Compared to the patient's condition at admission to the project, this patient's condition is: 1 - very much improved since the initiation of treatment; 2 - much improved; 3 - minimally improved; 4 - no change from baseline; 5 - minimally worse; 6 - much worse; 7 - very much worse since the initiation of treatment"

Trial Locations

Locations (1)

Burn Treatment Center, Dr. José Frota Institute

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Fortaleza, Ceará, Brazil

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