Effect of Red Raspberry Consumption on Fasting Oxidative Stress Biomarkers

Phase 1

Completed

• Conditions: Oxidative Stress
• Interventions: Dietary Supplement: individually quick frozen red raspberries

• Registration Number: NCT00876811
• Lead Sponsor: University of Toronto

Brief Summary

The purpose of this study is determine whether daily consumption of frozen red raspberries can improve the antioxidant capacity of the blood and reduce the effects of oxidative stress, dose-dependently, in a healthy adult population.

Detailed Description

Red raspberries are an excellent source of numerous polyphenolic antioxidants. The bioactivity of these compounds have been implicated in many biological pathways and may function to reduce the risk of numerous chronic diseases. We are conducting an intervention study with frozen red raspberries on a healthy adult population in order to determine if raspberry polyphenols are bioavailable and bioactive in vivo. Twenty-four subjects (12 male and 12 female) will be recruited to participate and consume one-cup frozen red raspberries daily for two weeks, they then will randomly be assigned to consume either one, two or four cups of raspberries for another two weeks, this will be followed by four weeks of non-treatment. Blood samples and 12-hour urine collections will be taken in order to measure the concentration of raspberry polyphenols in response to treatment dose. Dose-dependent treatment effects on blood biomarkers for antioxidant capacity, oxidative stress, inflammation and other chronic disease risk factors will also be measured.

Study Timeline

• Study Start: 2009-03
• Study First Submitted: 2009-04-03
• Study First Submitted QC: 2009-04-03
• Study First Posted: 2009-04-07
• Primary Completion: 2009-05
• Study Completion: 2009-05
• Status Verified: 2010-10
• Last Update Submitted: 2010-10-28
• Last Update Posted: 2010-10-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• healthy adults

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Exclusion Criteria

• obese (>30kg/m2)
• high blood pressure (140/90mmHg)
• allergic to raspberries
• pregnant or lactating
• history of chronic disease
• currently taking medication

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
two-cups	individually quick frozen red raspberries	-
one-cup	individually quick frozen red raspberries	-
four-cups	individually quick frozen red raspberries	-

Primary Outcome Measures

Name	Time	Method
serum TBARS	end of week 1, 3, 5 and 9	an indicator of MDA levels, and therefore, lipid peroxidation

Secondary Outcome Measures

Name	Time	Method
serum antioxidant capacity (AOC)	end of week 1, 3, 5 and 9	measured using the TEAC-ABTS radical cation assay
serum thiols	end of week 1, 3, 5 and 9	measured using the DTNB assay, as an indicator of antioxidant levels in protein fraction

Trial Locations

Locations (1)

Clinical Nutrition and Risk Factor Modification Centre, St. Michael's Hospital; 🇨🇦 Toronto, Ontario, Canada