Effect of Acute Red Raspberry Consumption on Post-prandial Oxidative Stress
- Conditions
- Oxidative StressAntioxidants
- Registration Number
- NCT01242033
- Lead Sponsor
- University of Toronto
- Brief Summary
Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 8
- healthy adults
- blood pressure greater than 120/80 mm Hg
- body mass index greater than 24.9 kg/m^2
- history of any chronic disease
- currently taking medications
- allergy to fruit
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Primary Outcome Measures
Name Time Method change in antioxidant capacity from baseline (-0.25 h) -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE)
- Secondary Outcome Measures
Name Time Method change in lipid peroxidation from baseline (-0.25 h) -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake serum thiobarbituric acid reactive substances (TBARS) measured as an indicator of the lipid peroxidation product malondialdehyde (MDA)
change in thiols compared to baseline (-0.25 h) -025, 0.25, 0.5, 1, 2, 4 hours post-intake plasma thiols quantified by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) assay as an indicator of the level of protection against protein oxidation
Trial Locations
- Locations (1)
Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital
🇨🇦Toronto, Ontario, Canada
Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital🇨🇦Toronto, Ontario, Canada