Hypertension and Injury
- Conditions
- CADArterial Hypertension
- Registration Number
- NCT02795377
- Lead Sponsor
- Klinik für Kardiologie, Pneumologie und Angiologie
- Brief Summary
Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial microparticles have been proposed as markers of endothelial injury. However, which mechanical forces contribute to their release is not clear.
- Detailed Description
In a first series subjects (50% hypertensives) with and without arterial hypertension and no Coronary Artery Disease (CAD) (n=50) will be recruited. MP subpopulations will be discriminated by flow cytometry according to the expression of established surface antigens including CD31+/41-, CD144+, and CD62e+. Besides office and ambulatory 24h blood pressure measurements, pulse wave analysis will be performed to determine central blood pressure, augmentation index (AIX), and pulse wave velocity. Endothelial function (Flow-mediated dilation, FMD), arterial pulsatile stretch (fractional diameter changes, FDC), and wall-shear-stress (WSS) will be measured in the same segment of the brachial artery (BA) by ultrasound. In a second series, the investigators will take measurements in subjects with hypertensive crises (SBP\>180 mmHg) (n=20) before and after 4h and normalization of arterial BP by urapidil. In a third series, the investigators will take measurement in subjects with stable CAD (n=10) before and after transfemoral coronary diagnostic angiography.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 80
- male subjects with or without arterial hypertension, hypertensive crises, stable CAD
- written informed consent
- subjects with manifest peripheral artery, or cerebrovascular disease, acute inflammation (CRP>0.6 mg/dl), malignancies, arrhythmias
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Changes in endothelial function Baseline and 4 hours after procedure endothelial function measured by flow-mediated dilation (FMD) for Subgroup 4 before and after transfemoral coronary diagnostic angiography
endothelial function Baseline endothelial function measured by flow-mediated dilation (FMD) for Subgroup 1 and 2
- Secondary Outcome Measures
Name Time Method Microparticle Baseline Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 1 and 2
Changes in Microparticle Baseline and after 4 hours Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 3
Changes Microparticle Baseline and 4 hours after procedure Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 4 before and after transfemoral coronary diagnostic angiography
Wall Shear Stress Baseline measured by ultrasound in brachial artery for Subgroup 1 and 2
Changes in Wall Shear Stress Baseline and 4 hours after procedure measured by ultrasound in brachial artery for Subgroup 4 before and after transfemoral coronary diagnostic angiography
changes in fractional diameter (FDC) Baseline and after 4 hours measured by ultrasound in brachial artery for Subgroup 3
Changes in fractional diameter (FDC) Baseline and 4 hours after procedure measured by ultrasound in brachial artery for Subgroup 4 before and after transfemoral coronary diagnostic angiography
pulse wave velocity Baseline measured for Subgroup 1 and 2
Changes in pulse wave velocity Baseline and 4 hours after procedure measured for Subgroup 4 before and after transfemoral coronary diagnostic angiography
augmentation index baseline measured for Subgroup 1 and 2
Changes in augmentation index Baseline and 4 hours after procedure measured for Subgroup 4 before and after transfemoral coronary diagnostic angiography
Trial Locations
- Locations (1)
Division of Cardiology, Pulmonology and Vascular Medicine Duesseldorf,
🇩🇪Duesseldorf, NRW, Germany