OPTImisation and Standardization of CIRCulating Cell-free DNA Ex-traction Protocols in a Diagnostic Setting

Not yet recruiting

• Conditions: Healthy

• Registration Number: NCT06869070
• Lead Sponsor: University Hospital Tuebingen

Brief Summary

comparison of blood collection tubes and cell-free DNA extraction to evaluate the best possible combination to obtain high quantity and quality cell-free DNA sample for NGS analyses

Detailed Description

Not available

Study Timeline

• Status Verified: 2025-02
• Study First Submitted: 2025-02-07
• Study First Submitted QC: 2025-03-06
• Last Update Submitted: 2025-03-06
• Study First Posted: 2025-03-11
• Last Update Posted: 2025-03-11
• Study Start: 2025-04-07
• Primary Completion: 2025-05
• Study Completion: 2025-08

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

• at least 18 years
• no serious condition

Exclusion Criteria

• younger than 18 years
• serious health condition that leads to higher cfDNA content in blood

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
cfDNA quantity	Baseline, at enrollment	we will assess the quantities in ng/µl of cell-free DNA extracted from plasma
cfDNA quality	Baseline, at enrollment	we will assess the fragments length in nucleotides using TermoFisher's Tapestation from the extracted plasma cell-free DNA