Multiomic Analysis of Traumatic Brain Injury and Hypertension Intracranial Hemorrhage Lesion Tissue
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Metabolite biomarkers in TBI or ICH patients' lesion tissues assessed by metabolomic bioinformatic analysis
研究概览
简要总结
The goal of this experimental observation study is to figure out differently expressed biomarkers in lesion tissues in traumatic brain injury or hypertension intracranial hemorrhage patients. The main questions it aims to answer is:
- Which RNA, protein and metabolites are differently expressed in lesion tissues?
- What molecular mechanism is participated in TBI or ICH? Participants will be treated by emergency operation, and their lesion tissues will be collected during the operation.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 16 Years 至 65 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age >18 and <65
- •Within 12 hours after brain injury
- •GCS ≥3 and ≤8
- •Closed brain injury with frontotemporal contusion or basal ganglia intracranial hemorrhage
- •Need emergency intracranial hematoma evacuating operation
- •Patient's agents are informed and consent the research
排除标准
- •With TBI, stroke, ICH or intracranial tumor history
- •Death within 24 hours
- •Immunosuppression state
- •Severe organ dysfunction
- •Complicated infective disease
结局指标
主要结局
Metabolite biomarkers in TBI or ICH patients' lesion tissues assessed by metabolomic bioinformatic analysis
时间窗: Immediately after collecting tissues
Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed by bioinformatic analysis to reveal different metabolite expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.
RNA biomarkers in TBI or ICH patients' lesion tissues assessed by transcriptome bioinformatic analysis
时间窗: Immediately after collecting tissues
Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed by bioinformatic analysis to reveal different RNA expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.
Protein biomarkers in TBI or ICH patients' lesion tissues assessed by proteomic bioinformatic analysis
时间窗: Immediately after collecting tissues
Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed by bioinformatic analysis to reveal different protein expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.
次要结局
未报告次要终点
研究者
Junfeng Feng
Director of Brain Injury Center
RenJi Hospital