Multiomic Analysis of Body Fluid in Acute Traumatic Brain Injury
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis
研究概览
简要总结
The goal of this experimental observation study is to figure out differently expressed biomarkers in body fluid in traumatic brain injury patients. The main questions it aims to answer is:
- Which RNA, protein and metabolites are differently expressed in lesion tissues?
- What molecular mechanism is participated in TBI? Participants will be treated by emergency operation, and their body fluid samples will be collected in emergency room and during the operation.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 16 Years 至 65 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age >18 and <65
- •Within 12 hours after brain injury
- •Control group is hydrocephalus
- •GCS ≥3 and ≤8
- •Closed brain injury with frontotemporal contusion
- •Need emergency intracranial hematoma evacuating or cerebrospinal fluid shunting operation
- •Patient's agents are informed and consent the research
排除标准
- •With TBI, stroke, ICH or intracranial tumor history
- •Death within 24 hours
- •Immunosuppression state
- •Severe organ dysfunction
- •Complicated infective disease
结局指标
主要结局
Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis
时间窗: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed and compared with control group to filter potential metabolite biomarkers in blood and cerebrospinal fluid of severe TBI patients.
RNA biomarkers in patients' blood & cerebrospinal fluid assessed by transcriptome bioinformatic analysis
时间窗: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed and compared with control group to filter potential RNA biomarkers in blood and cerebrospinal fluid of severe TBI patients.
Protein biomarkers in patients' blood & cerebrospinal fluid assessed by proteomic bioinformatic analysis
时间窗: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed and compared with control group to filter potential protein biomarkers in blood and cerebrospinal fluid of severe TBI patients.
次要结局
未报告次要终点
研究者
Junfeng Feng
Director of Brain Injury Center
RenJi Hospital