Multiomic Analysis of Body Fluid in Acute Traumatic Brain Injury
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Brain Injuries, Traumatic
- Sponsor
- RenJi Hospital
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis
- Status
- Recruiting
- Last Updated
- 3 years ago
Overview
Brief Summary
The goal of this experimental observation study is to figure out differently expressed biomarkers in body fluid in traumatic brain injury patients. The main questions it aims to answer is:
- Which RNA, protein and metabolites are differently expressed in lesion tissues?
- What molecular mechanism is participated in TBI? Participants will be treated by emergency operation, and their body fluid samples will be collected in emergency room and during the operation.
Investigators
Junfeng Feng
Director of Brain Injury Center
RenJi Hospital
Eligibility Criteria
Inclusion Criteria
- •Age \>18 and \<65
- •Within 12 hours after brain injury
- •Control group is hydrocephalus
- •GCS ≥3 and ≤8
- •Closed brain injury with frontotemporal contusion
- •Need emergency intracranial hematoma evacuating or cerebrospinal fluid shunting operation
- •Patient's agents are informed and consent the research
Exclusion Criteria
- •With TBI, stroke, ICH or intracranial tumor history
- •Death within 24 hours
- •Immunosuppression state
- •Severe organ dysfunction
- •Complicated infective disease
Outcomes
Primary Outcomes
Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis
Time Frame: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed and compared with control group to filter potential metabolite biomarkers in blood and cerebrospinal fluid of severe TBI patients.
RNA biomarkers in patients' blood & cerebrospinal fluid assessed by transcriptome bioinformatic analysis
Time Frame: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed and compared with control group to filter potential RNA biomarkers in blood and cerebrospinal fluid of severe TBI patients.
Protein biomarkers in patients' blood & cerebrospinal fluid assessed by proteomic bioinformatic analysis
Time Frame: Immediately after collecting blood & cerebrospinal fluid
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed and compared with control group to filter potential protein biomarkers in blood and cerebrospinal fluid of severe TBI patients.