Influence of Leucine Enriched Amino Acids on Myofibrillar and Collagen Protein Synthesis in Human Skeletal Muscle
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- Daniel Moore
- 入组人数
- 8
- 试验地点
- 2
- 主要终点
- Myofibrillar Protein synthesis
研究概览
简要总结
The primary objective will be to determine the effect of leucine-enriched essential amino acids (LEAA) compared to carbohydrate placebo on dietary incorporation of [D5] Phenylalanine & [D5] Glycine into the three skeletal muscle protein pools (myofibrillar, sarcoplasmic and collagen), both following resistance exercise and at rest, with the two tracers provided as a 'intrinsically labeled' bolus. Other outcomes will relate to molecular regulation of protein synthesis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Treatment
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
盲法说明
Double blinded (investigators and participants). A lab member external to the investigation will code items for the intervention.
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy, male and female, recreationally-active participants.
- •Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix B).
- •Self-reported not having engaged in resistance exercise and/or lower body plyometrics for at least 3 months prior to the study.
- •Participants will be aged 18-35 years old.
- •Participants are willing to abide by the compliance rules of this study.
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
排除标准
- •Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor.
- •Self-reported regular tobacco use.
- •Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
- •Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- •Use of birth control and discontinued use within the last 3 months (female participants).
结局指标
主要结局
Myofibrillar Protein synthesis
时间窗: 4 hours
Rate of incorporation of orally ingested amino acid tracers (D5 Phe and D5 Gly) into myofibrillar proteins isolated from skeletal muscle biopsies 4h following drink (LEAA or PLA) consumption and resistance exercise or at rest. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 4h period.
次要结局
- Collagen protein synthesis(4 hours)
- Sarcoplasmic protein synthesis(4 hours)
- Protein localization via immunofluorescence(4 hours)
研究者
Daniel Moore
Associate Professor
University of Toronto