Effect of Amino Acid on Muscle Damage

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Dietary Supplement: Active; Dietary Supplement: Placebo

• Registration Number: NCT03319147
• Lead Sponsor: University of Toronto

Brief Summary

The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise.

In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.

Detailed Description

Not available

Study Timeline

• Study Start: 2017-10-18
• Study First Submitted: 2017-10-18
• Study First Submitted QC: 2017-10-18
• Study First Posted: 2017-10-24
• Primary Completion: 2019-01-15
• Study Completion: 2019-10-30
• Status Verified: 2021-04
• Last Update Submitted: 2021-04-17
• Last Update Posted: 2021-04-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 20

Inclusion Criteria

• Healthy, male, recreationally-active participants
• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix. B). Active will be defined as being in the top 20% of age specific scores on iPAQ (the International Physical Activity Questionnaire; Appendix.C).
• Participants are required not to engage in lower limb RT (resistance training) for at least 6 months prior to the study.
• Participants will be 18-35 years old.
• Participants are willing to abide by the compliance rules of this study

Exclusion Criteria

• Female
• Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor
• Self-reported regular tobacco use
• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.)
• The individual who has allergy for milk

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Active	Active	4g of essential amino acids
Placebo	Placebo	4g of maltodextrin

Primary Outcome Measures

Name	Time	Method
Muscle protein synthesis	4 hours	Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-\[D5\]phenylalanine

Secondary Outcome Measures

Name	Time	Method
exercise performance	4 days	Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC).
Muscle soreness	4 days	Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm)
Muscle damage	4 days	Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming.

Trial Locations

Locations (1)

Goldring Centre for High Performance Sport; 🇨🇦 Toronto, Ontario, Canada