Influence of Leucine Enriched Amino Acids on Myofibrillar and Collagen Protein Synthesis in Human Skeletal Muscle
Trial Snapshot
- Phase
- Not Applicable
- Status
- Active, not recruiting
- Sponsor
- Daniel Moore
- Enrollment
- 8
- Locations
- 1
- Primary Endpoint
- Myofibrillar Protein synthesis
Study Overview
Brief Summary
The primary objective will be to determine the effect of leucine-enriched essential amino acids (LEAA) compared to carbohydrate placebo on dietary incorporation of [D5] Phenylalanine & [D5] Glycine into the three skeletal muscle protein pools (myofibrillar, sarcoplasmic and collagen), both following resistance exercise and at rest, with the two tracers provided as a 'intrinsically labeled' bolus. Other outcomes will relate to molecular regulation of protein synthesis.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Treatment
- Masking
- Triple (Participant, Investigator, Outcomes Assessor)
Masking Description
Double blinded (investigators and participants). A lab member external to the investigation will code items for the intervention.
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Healthy, male and female, recreationally-active participants.
- •Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix B).
- •Self-reported not having engaged in resistance exercise and/or lower body plyometrics for at least 3 months prior to the study.
- •Participants will be aged 18-35 years old.
- •Participants are willing to abide by the compliance rules of this study.
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
Exclusion Criteria
- •Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor.
- •Self-reported regular tobacco use.
- •Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
- •Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- •Use of birth control and discontinued use within the last 3 months (female participants).
Arms & Interventions
Leucine enriched essential amino acids
Leucine enriched essential amino acids (4g total, of which 1.6g are leucine). 20g carbohydrate, 2g of protein free drink powder, and 5g of Splenda to mask taste.
Intervention: Leucine enriched amino acids (Dietary Supplement)
Carbohdyrate Placebo
Iso-caloric placebo (4g maltodextrin to replace LEAA). 20g carbohydrate, 2g of protein free drink powder, and 5g of Splenda to mask taste.
Intervention: Carbohydrate Placebo (Dietary Supplement)
Outcomes
Primary Outcomes
Myofibrillar Protein synthesis
Time Frame: 4 hours
Rate of incorporation of orally ingested amino acid tracers (D5 Phe and D5 Gly) into myofibrillar proteins isolated from skeletal muscle biopsies 4h following drink (LEAA or PLA) consumption and resistance exercise or at rest. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 4h period.
Secondary Outcomes
- Collagen protein synthesis(4 hours)
- Sarcoplasmic protein synthesis(4 hours)
- Protein localization via immunofluorescence(4 hours)
Investigators
Daniel Moore
Associate Professor
University of Toronto