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Minimizing Toxicity in HLA-identical Sibling Donor Transplantation for Children With Sickle Cell Disease

Phase 2
Recruiting
Conditions
Sickle Cell Disease
Interventions
Drug: Alemtuzumab, low dose total body irradiation, Sirolimus
Registration Number
NCT03587272
Lead Sponsor
Robert Nickel
Brief Summary

This multisite prospective study seeks to determine if HLA-identical sibling donor transplantation using alemtuzumab, low dose total-body irradiation, and sirolimus (Sickle transplant Using a Nonmyeloablative approach, "SUN") can decrease the toxicity of transplant while achieving a high cure rate for children with sickle cell disease (SCD).

Detailed Description

This is a prospective, multicenter phase II study of HLA-identical sibling donor HSCT in 30 pediatric patients with SCD using nonmyeloablative conditioning with alemtuzumab, total-body irradiation, and sirolimus. The primary Objective of this study is to determine if the SUN regimen can decrease the incidence of grade II-IV acute graft-versus host disease (GVHD) by day +100 while maintaining similar disease-free survival compared to establish HLA-identical donor hematopoietic stem cell transplant (HSCT) regimens in children with SCD. The secondary Objective is to determine if health-related quality of life (HRQoL) for children undergoing SUN HSCT is preserved during the early post-transplant time period. To determine if the SUN regimen can decrease the number of platelet transfusions compared to established HLA-identical HSCT regimens in children with SCD. The tertiary/Exploratory Objectives: To describe other markers of toxicity (duration of neutropenia, mucositis, length of hospitalization) and indicators of a successful HSCT (HRQoL at 1 year, proportion needing additional immunosuppression during the first year, proportion able to wean sirolimus at 1 year).

With protocol version 5.0, added new secondary objective to determine if the change from intravenous to subcutaneous alemtuzumab and the addition of post-HSCT G-CSF can decrease the incidence of poor donor engraftment.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria

Not provided

Exclusion Criteria
  • General: Life expectancy less than 6 months. Pregnant or breastfeeding patients.
  • Infection Disease: Uncontrolled bacterial, viral or fungal infections (undergoing appropriate treatment and with progression of clinical symptoms) within 1 month prior to conditioning. Patients with febrile illness or suspected minor infection should await clinical resolution prior to starting conditioning. Patients with confirmed seropositivity for HIV and patients with active Hepatitis B or C determined by serology and/or NAAT are excluded.
  • Liver: Direct (conjugated) bilirubin > 1.5 mg/dL, transaminases >5x upper limit of normal for age.
  • Cardiac: Left ventricular shortening fraction <25% or ejection fraction <50% by ECHO.
  • Kidney: Estimated creatinine clearance less than 60 mL/min/1.73m2.
  • Pulmonary function: Diffusion capacity of carbon monoxide (DLCO) <35% (adjusted for hemoglobin). Baseline oxygen saturation <85% or PaO2 <70.
  • Heme: History of RBC alloantibodies against donor RBC antigens (even if current antibody screen is negative). Major ABO incompatibility with donor.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
SUN regimenAlemtuzumab, low dose total body irradiation, SirolimusAlemtuzumab, low dose total body irradiation, Sirolimus HLA-identical sibling donor transplantation using alemtuzumab, low dose total-body irradiation, and sirolimus (Sickle transplant Using a Nonmyeloablative approach, "SUN") can decrease the toxicity of transplant while achieving a high cure rate for children with sickle cell disease (SCD).
Primary Outcome Measures
NameTimeMethod
Acute GVHD100 days post transplant

Acute grade II-IV GVHD

Secondary Outcome Measures
NameTimeMethod
PedsQL 4.0 Measurement model for the Pediatric Quality of Life InventoryDay +30, and day +100 post transplant

PedsQL 4.0 assessments of health related quality of life before/after transplant

Poor donor engraftmentday +365

graft rejection, stem cell boost, or 1-year donor myeloid chimerism \<50%

Trial Locations

Locations (7)

Children's National Health System

🇺🇸

Washington, District of Columbia, United States

Ann & Robert H. Lurie Children's Hospital of Chicago

🇺🇸

Chicago, Illinois, United States

Columbia University

🇺🇸

New York, New York, United States

Levine Children's Hospital

🇺🇸

Charlotte, North Carolina, United States

Nationwide Children's Hospital

🇺🇸

Columbus, Ohio, United States

Alberta Children's Hospital

🇨🇦

Calgary, Alberta, Canada

The Hospital for Sick Children

🇨🇦

Toronto, Ontario, Canada

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