Innate Immune Response to Anaerobe and Aerobe Exercise in Rowing Athletes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- UMC Utrecht
- 入组人数
- 16
- 试验地点
- 2
- 主要终点
- Change in neutrophil surface marker expression profiles over time
研究概览
简要总结
Exercise induces innate immune response activation in athletes. The response starts during exercise and lasts for approximately 24 hours. This innate immune response shows similarities to the innate immune activation observed in, for example, bacterial infection and trauma. Immediately after exercise, athletes develop leukocytosis and cytokine production is altered towards an inflammatory pattern. However, to gain insight in immune response, a detailed cell receptor expression is required. To investigate the physiological innate immune response to exercise, we developed a model in rowers.
Aim: To determine the type and degree of cellular inflammatory response in peripheral blood of elite rowing athletes after both anaerobe and aerobe exercise.
Study design:
Investigator driven, monocenter observational pilot study.
Study population: 16 healthy, non-asthmatic, human volunteers, 18-25 year old. All competitive rowing athletes.
详细描述
Rationale: Exercise induces innate immune response activation in athletes. The response starts during exercise and lasts for approximately 24 hours. This innate immune response shows similarities to the innate immune activation observed in, for example, bacterial infection and trauma. Immediately after exercise, athletes develop leukocytosis and cytokine production is altered towards an inflammatory pattern. However, to gain insight in immune response, a detailed cell receptor expression is required. To investigate the physiological innate immune response to exercise, we developed a model in rowers.
Objective: To determine the type and degree of cellular inflammatory response in peripheral blood of elite rowing athletes after both anaerobe and aerobe exercise.
Study design: Investigator driven, monocenter observational pilot study.
Study population: 16 healthy, non-asthmatic, human volunteers, 18-25 year old. All competitive rowing athletes.
Intervention : All athletes perform two separate tests on a rowing ergometer. One anaerobe (flat out) exercise test (minutes) at day 1 and a aerobe test (1 hour) at day 2. Blood withdrawal of 9mL will be performed at four time points (1 pretest time point and 3 post: at 0h, 2 and 4h).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 25 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Elite rower: rowing for 12 months or longer, >6 times a week.
- •Aged 18-25 years
- •Pretested(by exercise test*) maximum heart rate
- •Visiting Rowing club Triton, Orca or Viking
- •Performed a sports medical examination test (required for competing on a national level by the Royal Dutch Rowing Asscociation) *The exercise test is a multi step power test which builds up to maximum effort.
排除标准
- •Asthmatic
- •Current respiratory infection, sinusitis, otitis or any other sign of acute/ chronic inflammatory disease
- •Physically injured
结局指标
主要结局
Change in neutrophil surface marker expression profiles over time
时间窗: pre, 0h, 2h and 4h
FACS analysis of peripheral blood neutrophils. The changes in surface markers over time will be analysed by principal component analysis.
次要结局
- Functional differences of neutrophil phenotypes before versus neutrophils that appear after exercise(pre and 2h post)
- Changes in absolute cell count(pre, 0, 2h and 4h)