Impact of Acute Exercise Intensity and Pattern on Cytokine Function

Not Applicable

Completed

• Conditions: Inflammation
• Interventions: Other: Moderate intensity continuous exercise; Other: Resting (no exercise) control; Other: High intensity continuous exercise; Other: High intensity interval exercise

• Registration Number: NCT05574413
• Lead Sponsor: University of British Columbia

Brief Summary

The immune system helps prevent illness, fights off infections, and repairs damaged tissues following an injury. However, when immune cells remain active for prolonged periods of time - a state known as "chronic inflammation" - they can contribute to the development and progression of chronic diseases like heart disease and diabetes. Exercise can reduce the risk of developing many of these diseases and at least part of the health benefits of exercise are due to the ability of exercise to reduce "chronic inflammation". The inflammation-lowering effects of exercise are typically captured by measuring hormone-like molecules released from immune cells called "cytokines" in the blood. In addition to changes in circulating cytokine levels, exercise may also alter how immune cells respond to these cytokines. How exercise intensity (i.e., how hard you are working during exercise) and pattern (i.e., exercising as a long continuous bout or in short intervals) impact the ability of immune cells to respond to cytokines is not well understood. A better understanding of how exercise intensity and pattern of exercise for reducing chronic inflammation may help determine the best types of exercises for improving health and preventing chronic diseases.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-09-15
• Study First Submitted: 2022-10-06
• Study First Submitted QC: 2022-10-06
• Study First Posted: 2022-10-10
• Primary Completion: 2023-08-01
• Study Completion: 2023-09-01
• Status Verified: 2023-12
• Last Update Submitted: 2023-12-04
• Last Update Posted: 2023-12-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 16

Inclusion Criteria

• 18-35 years of age
• Body mass index between 18.5-30 kg/m^2
• Free of cardiometabolic and autoimmune/inflammatory disease

Exclusion Criteria

• Competitive endurance athlete
• Cigarette smoker
• Currently taking immunomodulatory/anti-inflammatory medications
• Currently pregnant

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Moderate intensity continuous exercise (MICE)	Moderate intensity continuous exercise	Experimental session involving an acute bout of moderate intensity continuous exercise (MICE; continous cycling expending 350 kcal at 70% of lactate threshold)
Resting (no exercise) control	Resting (no exercise) control	Resting (no exercise) control condition
High intensity continuous exercise (HICE)	High intensity continuous exercise	Experimental session involving an acute bout of high intensity continuous exercise (HICE; continuous cycling expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)
High intensity interval exercise (HIIT)	High intensity interval exercise	Experimental session involving an acute bout of high intensity interval exercise (HIIT; cycling intervals expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)

Primary Outcome Measures

Name	Time	Method
IL-10 mediated STAT3 phosphorylation	Change from pre-exercise to immediately and 90-min post-exercise	Ex vivo leukocyte STAT3 phosphorylation in response to IL-10 treatment

Secondary Outcome Measures

Name	Time	Method
IL-6 mediated STAT3 phosphorylation	Change from pre-exercise to immediately and 90-min post-exercise	Ex vivo leukocyte STAT3 phosphorylation in response to IL-6 treatment
Plasma TNF-alpha	Change from pre-exercise to immediately, 30-, and 90-min post-exercise	Concentration of TNF-alpha in plasma samples
Hematology panel	Change from pre-exercise to immediately, 30-, and 90-min post-exercise	Complete blood count
Plasma IL-10	Change from pre-exercise to immediately, 30-, and 90-min post-exercise	Concentration of IL-10 in plasma samples
IL-10 mediated TNF-alpha inhibition	Change from pre-exercise to immediately and 90-min post-exercise	Ex vivo inhibition of TNF-alpha production in response to IL-10 treatment
Extracellular vesicles	Change from pre-exercise to immediately, 30-, and 90-min post-exercise	Concentration of extracellular vesicles in plasma
IL-6 mediated TNF-alpha inhibition	Change from pre-exercise to immediately and 90-min post-exercise	Ex vivo inhibition of TNF-alpha production in response to IL-6 treatment
Plasma IL-6	Change from pre-exercise to immediately, 30-, and 90-min post-exercise	Concentration of IL-6 in plasma samples

Trial Locations

Locations (1)

UBC Okanagan; 🇨🇦 Kelowna, British Columbia, Canada